Formulation and Evaluation of Herbal Extract of Butterfly Pea Multipurpose Cream

 

Pradip D. Dhangar*, Harshal Shimpi, Rohit Newadkar, Vivek Bhadane,

Lupesh Desale, Neha Jaiswal

Department of Pharmaceutics, Ahinsa Institute of Pharmacy, Dondaicha Dist. Dhule.

*Corresponding Author E-mail: dhangarpradip225@gmail.com.

 

ABSTRACT:

Natural remedies are more acceptable and safer than synthetic ones. The main aim of our study is To Develop a herbal cream which can give multipurpose effect, like Moisturizer, reduce acne and skin Irritation, reduce skin problems Like eczema, psoriasis, dry skin, wrinkles, rashes etc. and also Adding Glow to the face. We have used three herbal ingredients in our Preparation which are Butterfly pea flowers, Aloe Vera gel, Neem, Aloe Vera gel. This indicates the presence of Anthocyanins, Butterfly pea flowers contain anthocyanins, which are natural antioxidants that slow Down the aging process. Prevents skin aging Neem, Aloe Vera gel is Used as a moisturizer, to reduce Pimples and acne and also used for Treatment of burn wounds. Neem is used as an antifungal and Anti-inflammatory and it is also used to reduce scar, pigmentation, Redness and itching of the skin .are some of the basic drugs used to formulate the cream. The selection of ingredients based on the Different medicinal properties of the agents. In quality evaluation of Formulation (F1-F3) were done On different parameters like PH, Viscosity, spreadability and phase separation. F2 is better formulation Than F1 and F3 of formulation of Multipurpose herbal cream. There were no change in physical Properties of multipurpose cream. The formulation showed good spreadabiliy, no phase separation and Good consistency. It is found that viscosity of the cream is Adequate. Thus herbal cosmetics Formulation is safe to use was stable at room temperature And can be safely used on the skin.

 

KEYWORDS: Herbal Cream, Butterfly pea, Aloe barbadensis (gel), Azadirachta indica (Neem), Herbal cosmetic, Multipurpose cream

 

 


INTRODUCTION:

Cream is defined as semisolid emulsions which are oil in water (o/w) or water in oil (w/o) type and these semisolid emulsions are Intended for external application.  Cream is classified as oil in Water and water in oil emulsion. It is applied on outer part or Superficial part of the skin and its main ability is to remain for a Longer period of time at the site of application. The function of a skin Cream is to protect the skin against different environmental Condition,

weather and gives soothing effect to the skin. There are Different types of creams like cleansing, cold, foundation, vanishing, Night, massage, hand and body creams. The main aim of our work is To develop a herbal cream which can give multipurpose effect, like Moisturizer, reduce acne and skin irritation, reduce skin diseases Like eczema, psoriasis, dry skin, wrinkles, rashes etc. and also adding Glow to the face. We have used three herbal ingredients in our Preparation which are Butterfly pea, Aloe Vera gel, Neem, Aloe Vera gel is Used as a moisturizer, to reduce pimples and acne and also used for Treatment of burn wounds. Neem is used as an antifungal and Anti-inflammatory and it is also used to reduce scar, pigmentation, Redness and itching of the skin.1

The butterfly pea or Clitoria ternatea L. is a member of the Fabaceae family. The blue color of Butterfly Pea flowers. This indicates the presence of anthocyanins. Depending on the application, it is used to color food or other objects. The butterfly pea (Clitoria ternatea L.) is one of those plants in which all parts have a beneficial effect on our body. The flowers contain polyacylated anthocyanins and flavonol glycosides called ternatins. These include antioxidants, antidiabetic agents, ant obesity agents, antiinflammatory agents, anticancer agents, antihyperlipidemic agents, and antiasthmatic agents. It has a positive effect on health. This corresponds to the fact that anthocyanins are easily soluble in water due to their chemical structure. Butterfly pea flowers contain anthocyanins, which are natural antioxidants that slow down the aging process. Prevents skin aging and helps the skin.2,3,4

 

MATERIALS AND METHODS:

1)    SELECTION OF RAW MATERIALS:

The materials for a formulation were selected on the basis study of different brief literature review and surfing of general and publication.

 

2)    COLLECTION OF RAW MATERIALS: 

Neem Azadirachta indica leaves and Tulsi leaves Ocimum sanctum were collected from local area from Dondaicha and Butterfly pea flowers were collected from local area from Anarad.

 

3)    EXCIPIENTS AND HERBAL INGREDIENTS WITH THEIR ROLE:

Table1:- Role of Ingredients 

Sr. No

Ingredients 

Role

1.

Butterfly Pea Flowers Extract

Antioxidants, Anti-inflammatory

2.

Aloe Vera gel

Anti-ageing, anti-inflammatory, moisturizer, reduce acne and pimples.

3.

Neem extract

Promote wound healing, relieves skin dryness, itching and redness.

4.

Beeswax

Emulsifying agent, stabilizer and gives thickness to the cream.

5.

Liquid paraffin

Lubricating agent

6.

Borax

Alkaline agent which reacts with emulsifying agent to form soap

7.

Methyl paraben

Preservative

8.

Rose water

Fragrance

 

4)    Extraction processes :

Anthocyanin extraction From Butterfly pea Flowers: 

Hot water extraction: Petals of fresh blue pea flowers were blended with water in a specific substrate solvent ratio [1:15 or 1:20 (w/v)] using a commercial blender, at low speed for 40 s. Next, the blended mixture was placed in a thermostatic water bath set at a specific temperature (40, 50 or 60°C) for a specific time period (30 or 60 min) while shaking. Then the mixture was filtered and dried at 40°C for 36 to 48h. The vacuum-dried extracts were then referred as anthocyanin extracts. Optimum treatment conditions selected from hot water extraction were used for subsequent ultrasound, microwave and pectinase-assisted extractions.7

 

Fig1: Dried Butterfly pea flowers

 

Fig 2:  Extraction Process

 

Confirmatory Tests for Anthocyanin: 

The confirmatory Tests for anthocyanin were performed using standard Procedures.

 

Sulfuric acid test:

1mL of concentrated H2SO4 was added To 2mL extract. The presence of anthocyanin would be indiCated by orange coloration of the interface.

 

Sodium hydroxide test:

2 drops of 1 N NaOH were added To 2mL of extract. The presence of anthocyanin would be Indicated by blue to bluish-green coloration.

 

Fig 3: Confirmatory Test for Anthocyanin

Extraction of Aloe Vera gel:

Mature, healthy and fresh aloe Vera leaves were collected and washed with distilled water. Then after proper drying of leaves in Hot air oven, the outer part of the leaf was dissected longitudinally using a sterile knife. Then the aloe Vera gel that is the colorless Parenchymatous tissue was removed using the sterile knife. Then it is filtered using muslin cloth to remove the fibers and impurities. Then the filtrate or the filter product which is a clear aloe Vera gel was used in the preparation.

 

Extraction of neem leaves:

Neem leaf was dried in an Oven at 50°C and prepared by blending 50g of the dried Leaf with 100ml of methanol for 10min. The crude extract Was filtered through muslin followed by Whatman No. 1 Filter paper prior to autoclaving (121°C for 15 mins) before Storage at -20°C

 

Fig 4: Herbal Extracts Butterfly pea flowers

 

Fig 5: Alov vera

 

Fig 6:  Neem

 

6) FORMULATION OF CREAM:

Heat liquid paraffin and beeswax in a borosilicate glass beaker at 75 and maintain that heating temperature. (Oil phase). In another Beaker, dissolve borax, methylparaben in distilled water and heat. This beaker to 75 to dissolve borax and methylparaben and to get A clear solution. (Aqueous phase).

 

Then slowly add this aqueous Phase to heated oily phase7. Then add a measured amount of aloe Vera gel, Neem extract, and Tulsi extract and stir vigorously until it Forms a smooth cream. Then add few drops of rose oil as a fragrance. Put this cream on the slab and add few drops of distilled water if Necessary and mix the cream in a geometric manner on the slab to Give a smooth texture to the cream and to mix all the ingredients Properly. This method is called as slab technique or extemporaneous Method of preparation of cream. (For formulation table refer table 2) (For different cream formulations refer fig. 8).

 

Fig 7: Slab and spatula

 

Fig 8: Formulations Process of Cream

 

Table 2: Formula Table For Cream:  

Sr. No

Ingredients

F1

F2

F3

1.

Butterfly Pea Flowers Extract 

1.5ml

1ml

1ml

2.

Aloe Vera gel

1.5ml

1ml

1ml

3.

Neem extract

0.5ml

0.2ml

0.4ml

4.

Beeswax

3g

3.5g

3.2g

5.

Liquid paraffin

10ml

15ml

12ml

6.

Borax

0.2g

0.4g

0.3g

7.

Methyl paraben

0.02g

0.04g

0.03g

 

Fig 9: Formulation of Multipurpose Herbal Cream

 

Fig 10: Formulation cream in different Composition F1, F2 and F3

 

EVALUATION OF CREAM:   

Physical evaluation:

In this test, the cream was observed for color, odor, texture, state (table 3) 

 

Irritancy:

Mark the area (1 cm2) on the left-hand dorsal surface. Then the cream was applied to that area and the time was noted. Then it is checked for irritancy, erythema, and edema if any for an interval up to 24 h and reported (table 4).

 

Wash ability:

A small amount of cream was applied on the hand and it is then washed with tap water (table 5).

 

PH:

0.5g cream was taken and dispersed in 50ml distilled water and then PH was measured by using digital PH meter (table 6) 

 

Viscosity:

Viscosity of cream was done by using Brooke field viscometer at a temperature of 25 using spindle No. 63 at 2.5RPM (table 7). 

 

Phase separation:

Prepared cream was kept in a closed container at a temperature of 25 - 100 away from light. Then phase separation was checked for 24h for 30d. Any change in the phase separation was observed/checked (table 8). 

 

Spread ability:

The spreadability was expressed in terms of time in seconds taken by two slides to slip off from the cream, placed in between the slides, under certain load. Lesser the time taken for separation of the two slides better the spreadability. Two sets of glass slides of standard dimension were taken. Then one slide of suitable dimension was taken and the cream formulation was placed on that slide. Then other slide was placed on the top of the formulation. Then a weight or certain load was placed on the upper slide so that the cream between the two slides was pressed uniformly to form a thin layer. Then the weight was removed and excess of formulation adhering to the slides was scrapped off. The upper slide was allowed to slip off freely by the force of weight tied to it. The time taken by the upper slide to slip off was noted. (table 9)

 

Spread ability = m × l/t 

Where, 

M= Standard weight which is tied to or placed over the upper slide 

(30g) 

L= length of a glass slide (5cm) 

T= time taken in seconds. 

 

Greasiness:

Here the cream was applied on the skin surface in the form of smear and checked if the smear was oily or grease-like (table 10)                    

 

RESULTS AND DISCUSSION:

Evaluation results of all the 3 formulations are gives below. 

Physical evaluation:

In this test color, odor, texture and state of the three formulations were examined. 

 

Table 3: In this test colour odor, texture and state of the three formulations was checked

S. No. 

Parameters Formulation

F1 Formulation

F2 Formulation

F3 Formulation

1.

Colors

Green

Faint Blue 

White 

2.

Odor

Pleasant

Pleasant

Pleasant

3.

Texture

Smooth

Smooth

Smooth

4.

State

Semisolid

Semisolid

Semisolid

 

Irritancy:

Mark the area (1 cm2) on left hand dorsal surface. Then the cream was applied to that area and the time was noted. Then it is checked for irritancy, erythema, and edema if any for an interval up to 24h and reported. According to the results all the three formulations that Is F1H, F2H and F3H showed no sign of irritancy, erythema and Edema.

 

Table 4: Irritancy study observations: 

S. No

Formulation

Irritant Effect

Erythema

Edema

1.

F1

Nil

Nil

Nil

2.

F2

Nil

Nil

Nil

3.

F3

Nil

Nil

Nil

 

Washability:

Washability test was carried out by applying a small amount of Cream on the hand and then washing it with tap water. All three formulations were easily washable.

 

Table 5: Washability observations

S. No.

Formulation

Wash ability

1.

F1

Easily Washable

2.

F1

Easily Washable

3.

F1

Easily Washable

 

pH:

According to the results, the PH of all the three formulations that is F1H, F2H andF3H were found to be nearer to skin PH so it can be safely used on the skin.

 

Fig 11: PH

 

Table 6: pH observation table

S. No

Formulation

PH

1.

F1

7.0

2.

F2

7.15

3.

F3

7.18

 

Viscosity:

Viscosity of cream was done by using Brooke field viscometer at a Temperature of 25 using spindle No. 63 at 2.5 RPM. According to The results all the three formulations showed adequate viscosity.

 

Table 7: Viscosity observation table

S. No

Formulation

Viscosity(Cps)

1.

F1

21020

2.

F2

11810

3.

F3

18820

 

Phase separation:

Prepared cream was kept in a closed container at a temperature of 25-100 away from light. Then phase separation was checked for 24 h for 30 d. Any change in the phase separation was observed/checked. According to the results no phase separation was observed in all the Three formulations

 

Table 8: Phase separation observation table

S. No

Formulation

Phase separation

1.

F1

No Phase separation

2.

F2

No Phase separation

3.

F3

No Phase separation

 

Spreadability:

The spreadability of the three formulations that is F1, F2, and F3 was carried out and out of that for F2 the time taken by the 2 Slides to separate is less so as said in the description of evaluation Test lesser the time taken for separation of the two slides better the Spreadability so according to this statement F2 showed better Spreadability.

 

 

 

 

Table 9: Spreadability observation table

S. No

Formulation

Time (sec)

Spread ability (g×cm/sec)

1.

F1

6

 4.16

2.

F2

7

 3.57

3.

F3

10

 2.5

 

Greasiness:

Here the cream was applied on the skin surface in the form of smear and checked if the smear was oily or grease-like. According to the Results, we can say that all three formulations were non-greasy.

 

Table 10: Greasiness observation table

S. No

Formulation

Greasiness

1.

F1

Non-greasy

2.

F2

Non-greasy

3.

F3

Non-greasy

 

CONCLUSION:

Nowadays herbal cosmetics one gaining popularity. Among customers These is a growing demand for herbal cosmetics in the world market and They are invaluable Gifts of Nature. The herbal multipurpose cream was Prepared from various herbal extract and have different significant activity in formulation. The various parameter of herbal multipurpose Cream Was Performed such as pH, Viscosity, speadability, phase separation, irritancy and good consistency, Base on the result f2 is shown proper physical Characteristics as compare to f1 and F3. The present work focus on the Potential of Herbal Extract form Cosmetic Purpose. The use bioactive Ingredient in cosmetic influence biological function of skin and provide Nutrient necessary for healthy skin. the Prepare formulation show good Speadability, no phase separation and good consistency during the Study Period, Base on the result and disassociation the formulation f2 is to it Better as compere F1 and F3

 

REFERENCES:

1.      Nikhil Nitin Navindgikar, K. A. Kamalapurkar, Prashant S. Chavan.  Formulation and evaluation of multipurpose herbal cream. International Journal of Current Pharmaceutical Research. 2020; 3(12). DOI: http://dx.doi.org/10.22159/ijcpr.2020v12i3.38300.

2.      Araya Suebkhampet, Pongsiwa Sotthibandhu. Effect of using aqueous crude extract from butterfly pea flowers (Clitoria ternatea l.) as a dye on animal blood smear staining, Suranaree J. Sci. Technol. 2012;19(1).  

3.      Jesslyn Sofyan, Tabligh Permana, Abdullah Muzi Marpaung. The Study of Several Applicable Treatments for Serving Butterfly Pea Flower Drinks, Advances in Biological Sciences Research. 2021; 16.

4.      Nur Amira Abd Rashid, Angzzas Sari Mohd Kassim, Aisyah Mohamed Rehan1. Evaluation of Butterfly Pea Flowers for Antioxidant Activity and its Potential as Antioxidant Soap. publisher.uthm.edu.my/periodicals/index.php/peat, vol. 3, no. 1, 2022, doi.org/10.30880/peat.2022.03.01.008. 

5.      R. N. Shah, B.M. Methal, (2006) A Handbook of Cosmetic, Vallabh Prakashan.

6.      Anuradha Keshwar, Unmesh Keshwar, Ashwini Deogirkar, S. S. Dhurde, Veena Deo and B. K. Shrikhande, Formulation Development and Evaluation of Cream Containing Natural Essential Oils having Mosquito Repellent Property. World Journal of Pharmacy and Pharmaceutical Sciences. 5(8): 1586-1593.

7.      Gayan Chandrajith Vidana Gamage, Wee Sim Choo. Hot water extraction, ultrasound, microwave and pectinase-assisted extraction of anthocyanins from blue pea flower.  Food Chemistry Advances. 2023; 2.  

8.      Sk Uddandu Saheb, Aduri Prakash Reddy, K Rajitha, B Sravani, B Vanitha. Formulation and evaluation of cream from naturally Containing plant extracts. World J Pharm Pharm Sci 2018;7:851-62.

9.      Bhakti Mali, Sumedh N Moharil, Vaibhav Mhasal, Mahesh B Narkhede. Drugexcipient interaction study of Tramadol HCL With polymers. World J Pharm Res. 2017; 6: 84861.

10.   Ricarl Irish F. Agunos, Danilet Vi M. Mendoza, and Michael Angelo S. Rivera1. Anthocyanin Colorimetric Strip for Volatile Amine Determination. International Journal of Food Science. 2020. https://doi.org/10.1155/2020/1672851. 

11.   Hany M. Yehia. Methanolic Extract of Neem Leaf (Azadirachta indica) and its Antibacterial  Activity Against Foodborne and Contaminated Bacteria on Sodium Dodecyl  Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). American-Eurasian J. Agric. & Environ. Sci.

12.   Archana Dhyani, Vikas Chander, Dr. Nardev Singh. Formulation and Evaluation of Multipurpose Herbal Cream. Journal of Drug Delivery and Therapeutics. 2019; 2(9): http://dx.doi.org/10.22270/jddt.v9i2.2540. 

13.   Surya Prabha. Matangi, Santhosh Aruna. Mamidi, Gulshan.   Raghavamma, Rama Rao Nadendla. Formulation and Evaluation of Anti-Aging Poly Herbal Cream. International Journal of Pharmaceutical Sciences Review and Research. 2020; 2(24): www.globalresearchonline.net. 

14.   Ashwini S. Dhase, Somishwar S. Khadbadi and Shweta S. Saboo. Formulation and Evaluation of Vanishing Herbal Cream of Crude Drugs. American Journal of Ethnomedicine. 2014; 5(1): http://www.ajethno.com. 

15.   Somnath S Davkhar, Aarti S Bhandari, Sanjivani A Akolkar. Formulation and Evaluation of Multipurpose Herbal Cream. 2023; 1(14). DOI: 10.31858/0975-8453.14.1.23-28. 

16.   R. Chandrasekar, B. Sivagami. Formulation and Evaluation of a Poly Herbal Skin Care Cream containing Neem and Tulsi. Research J. Topical and Cosmetic Sci. 2018; 9(1): DOI: 10.5958/2321-5844.2018.00006.7. 

17.   Piya Kosai, Kanjana Sirisidthi, Kanitta Jiraungkoorskul and Wannee Jiraungkoorskul. Review on Ethnomedicinal uses of Memory Boosting Herb, Butterfly Pea, Clitoria ternatea. Journal Of Natural Remedies. 2015; 2(15).

18.   Akash S. Mali, Karekar P, Yadav A. V. Formulation and Evaluation of Multipurpose Herbal Cream. International Journal of Science and Research (IJSR). 2015; 11(4). www.ijsr.net. 

 

 

 

 

 

 

Received on 12.08.2023         Accepted on 06.09.2023              

Accepted on 07.10.2023        ©A&V Publications all right reserved

Research J. Topical and Cosmetic Sci. 2023; 14(2):85-90.

DOI: 10.52711/2321-5844.2023.00013