An Overview of Stem Cell Therapy
Kunal D. Sutar*, Rutika D. Harshad*, Omkar S. Sangar, Aishwarya C. Patil, Santosh A. Payghan
Vasantidevi Patil Institute of Pharmacy, Kodoli MS.
*Corresponding Author E-mail: kkunalsutar@gmail.com
ABSTRACT:
KEYWORDS: Mesenchymal stem cells, stem cells, cell-based therapies.
INTRODUCTION:
The human body is made up of about 200 specialized cells, such as muscle cells, nerve cells, fat cells, and skin cells. All special cells come from stem cells. A stem cell is an unselected cell. Different types of stem cells have different levels of potency. The stem cell of almost every cell in the body is called a pluripotent and the stem cell of only certain cell types is called multipotent1.
Stem cells are defined as cells with clonogenic and regenerative capacity and divide many cell lines. Stem cells are found in all of us, from the earliest stages of human growth to the end of life. Stem cells that are unknown cells grow into specialized cells that form different types of tissue in the human body. Scientists have recently come to understand stem cells well enough to consider the possibility of long-term growth outside the body. At that early stage, more difficult tests can be performed, and it is possible to exploit these cells in such a way that certain tissues can grow literally2.
Figure 1: Type of stem cells1
Stem cells are the basic cells of all the organs and tissues in our body. The very special cells that make up these tissues originally came from the first pool of stem cells that were formed shortly after conception. Throughout our lives, we continue to rely on stem cells to replace damaged tissue and cells that are lost every day, such as those in the skin, hair, blood and intestinal tract.
In 1966, intraperitoneal distribution chambers implanted with rat bone marrow cells showed that isolated “stem” cells were present and led to osteogenic concentrations of alkaline phosphatase (AlkP) cells and fibroblasts during hematopoietic cells.
Cells are capable of building all the tissues in the human body, which is why they have great potential for future medical use in tissue regeneration and repair. For cells to fit within the definition of "stem cells," they must reflect two important characteristics. First, stem cells must be able to reproduce indefinitely to produce exactly the same as the emerging cell. This feature also applies to cancer cells that divide in an uncontrolled way and stem cell division is highly regulated. Therefore, it is important to note the additional requirement for stem cells; they must be able to provide a special cell type that becomes part of a healthy animal5. It is essential for growth, development, maintenance and repair of the brain, bones, muscles, nerves, blood, skin, and other organs. While stem cell-based therapies have been developed as standardized care, such as hematopoietic stem cell transplants for leukemia and epithelial stem cell-based treatment of burns and corneal disorders, the prevalence of cell-based therapies has increased in recent years as a result of advances. . in stem cell research.
In 2006, researchers made further progress by identifying conditions that would allow certain specialized cells to be “genetically modified” for a stem cell. This is a new stem type called stem pluripotent stem cells (IPSCs)6. At that time there may still be a difficult test, and it is possible to use these cells in such a way that certain tissues can actually grow.
The common term, "cell stem" includes many types of cells. Normally, modifiers, "embryonic," and "adult" are used to classify stem cells by the growth stage of the animal from which they originated, but these terms are not sufficient as new research has found how to regenerate old cells completely separated from embryonic stem cells, and , stem stem cells, aptly called "somatic" stem cells meaning "in the body", are found in the fetus, brain, spinal cord and in infants. Thus, stem cells are divided into two groups based on their biologic genes - pluripotent stem cells and multi-stem cells. Their sources, features, differences and treatment requests are discussed.1
Pluripotent stem cells are so called because they have the ability to isolate all kinds of cells in the body. In ecological development, pluripotent stem cells are found only for a very short time in the embryo before being separated into many specialized cells that eventually donate specialized tissue. These are limited numbers of cellular cells that come in many forms: some may be cells of a particular type of virus (endoderm, mesoderm, ectoderm) and others, only cells of a particular tissue.1
SITE TREATMENT RESOURCES:
Embryonic stem cells:
Embryonic stem cells of pluripotent recurrent cells may be mortally7. They are found in fetuses in the developmental stage before fertilization usually takes place in the uterus.
Large stem cells:
Old stem cells are totipotent or multipotent cells that cannot be separated. Unlike embryonic stem cells, which are described in terms of their origin, the origin of large stem cells in certain mature tissues is still being investigated9.
Stem cell types:
They are divided into two groups, embryonic and non-embryonic (known as large stem cells). In terms of stem cell division it is divided into 5 types: totipotent, pluripotent, multipotent, oligopotent and unipotent10.
Powerful particle cells can be classified into S Jagiri et al Asian Journal of Pharmaceutical Research and Development. 2019; 7 (5): 92-102 ISSN: 2320-4850 [93] CODEN (USA): AJPRHS embryos and extraembryonic cell types. Pluripotent stem cells produce any type of endoderm, mesoderm and ectoderm cell, and most stem cells differ from any other type of cell family closely related. Oligopotent and weak cells have the ability to differentiate between several cell types and only one cell type.
Totipotent The ability to differentiate into all possible cell types. Examples are zygote, which is made up of fertilized eggs and the first few cells from the zygote.
Pluripotent The ability to differentiate almost all cell types. Examples include embryonic stem cells and cells found in mesoderm, endoderm, and ectoderm germ molecules formed in the early stages of embryonic stem cell1 differentiation.
Multipotent The ability to differentiate into a closely related family of cells. Examples include hematopoietic (adult) cells which can be red and white blood cells or platelets1.
Oligopotent ability to divide into a few cells. Examples include (adult) lymphoid or myeloid stem cells.
Not only do they have the ability to produce cells of their own kind, but they also have the regenerative properties that need to be labelled. Examples include (adult) stem cell cells11.
Classification of stem cell on basis of potency
STEM CELL THERAPY:
1) Pluripotent stem cells:
Pluripotent stem cells have not been used therapeutically in humans because many early animal studies led to the unpleasant formation of abnormal hard tissue, called teratomas. Teratomas are formed by the combination of cell types in all layers of the original virus. Successful animal studies later used pluripotent cells that were transformed into a more mature phenotype that inhibited this growth. Cells are taken from many cells that have been used successfully to treat animals. For example, diabetic animals are designed to develop glucose-producing cells that respond to glucose levels. Also, animals with severe spinal cord injury or visual impairment were treated with the formation of new myelinated neurons or retinal epithelial cell cells, respectively. Commercial companies are currently in talks with the FDA regarding the possibility of advancing human trials. Other animal studies are designed to treat a wide range of ailments, such as Parkinson's disease, muscular dystrophy, and heart failure1.
The pluripotent stem cells used in research today come mainly from embryos, hence the name, "embryonic stem cells". Premature embryos that are only a few days old contain only 10-15% pluripotent cells in the "cell mass" (Figure 1). Those pluripotent cells can be separated, and then enlarged on the edge of the "feeder" cells providing unknown indicators of multiple growth cycles while maintaining their maximum energy 1.
2) Multipotent stem cells:
Multiple stem cells can be an effective means of clinical use. These cells are plastic and therefore all are ancestral cells of a specific viral layer or can be prevented from becoming one or two special cell types of certain tissues. Most highly differentiated stem cells are found in the developing fetus during digestion (day 14-15 in humans, day 6.5-7 in mice). These cells produce all the cells of their virus; therefore, they are still flexible in their ability to differentiate. They are not pluripotent cells because they have lost the ability to be cells of all three layers of the virus (Figure 1). At the lower end of the plasticity spectrum there are weak cells that can be only one type of cell such as stem cells or muscle stem cells. These stem cells are found inside their organs and although their ability to differentiate is restricted, these unlimited parent cells play an important role in maintaining tissue integrity by replenishing old or damaged cells. There are many subspecies of particle cells that have many types of differentiation. For example, cells with multiple cells found in the abdominal mesoderm experience a dividing action that reduces body and connective tissue; however, the additional difference leads to a special increase in the target only of the connective tissue and so on until the cells are able to produce only cartilage or bone1.
Many of the stem cells found in the bone marrow are well-known, since these have been used in medicine since the 1960's 10 (their power will be discussed in more detail in the next section). Recent research has found new sources of muscle cells such as the placenta and umbilical cord blood. In addition, the heart, which is still considered stem cells, is now known to contain stem cells that have the potential to become myocytes of the heart. Similarly, neuro-progenitor cells have been found in the brain.
Heart cells exist in such small numbers, that they are difficult to read and have never worked completely determined1.
APPLICATIONS OF STEM CELLS:
The purpose of any stem cell treatment is to repair damaged tissue that cannot heal itself. Ongoing research on stem cell therapy offers hope to patients who rarely receive antiretroviral therapy but simply reduce the symptoms of chronic illness. Stem cell therapy involves more than simply placing cells in the body and directing them to the growth of new, healthy tissue. It is also possible for coax stem cells already in the body to work overtime and produce new tissue.
1) Possible Treatment for Stem Cells:
There are several treatments for stem cells, but most are in the stages of testing and / or costly, excluding bone marrow transplantation. Medical researchers expect stem cells and the elderly to treat cancer, Type 1 diabetes mellitus, Parkinson's disease, Huntington's disease, Celiac disease, heart failure, muscle and nerve damage, and much more12. They suggested that before stem cell therapeutics are used in a clinical setting, further research is needed to understand the effectiveness of stem cell transplantation and the mechanisms of communication between stem cell and infected/damaged microenvelo.
Bone marrow transplantation (BMT) is a well-known clinical program for stem cell transplantation. BMT can replenish the bone marrow and restore all types of blood cells after high-dose chemotherapy and/or radiotherapy, our main protection used to eradicate cancer cells.
Possible treatment by stem cells
2) Skin replacement:
The knowledge of stem cells has enabled scientists to grow skin from a patient's cut hair. Keratinocyte stem cells reside in the hair follicle and can be removed during hair removal.
3) Brain cell transplantation:
Stem cells can produce dopamine - a drug that has no victims of Parkinson's disease. It involves the loss of cells that produce the neurotransmitter dopamine. The first blind study of stem cell transplantation in Parkinson's disease reported the survival and release of dopamine into transplanted cells and improved clinical signature function13. However, some patients have side effects, suggesting that they are overly sensitive or have too much dopamine. Although side effects are not expected, the success of cellular testing is significant.
4) Stem Cell Therapy for HIV:
The design of the hematopoietic stem has long been considered a victim of HIV (HIV) infection that inhibits the production of immune cells. The latest data document eliminates the dysfunction of HIV cells and shows that the immune system is driven by a new generation of T-cells. The effects of HIV on stem cell physiology, however, appear to be indirect, as stem cells are highly resistant to HIV infection. Although there are surface receptors for HIV, the hematopoietic stem cell is not infected with HIV and can act as a treatment for AIDS.
5) Veterinary medicine:
Research on horses, dogs, and cats can benefit from the development of stem cell therapies in medicine and could point to a variety of injuries and diseases such as myocardial infarction, stroke, tendon and ligament damage, osteoarthritis, osteochondrosis and muscular dystrophy in both large animals, and in humans. While research into cell-based therapies has often shown the need for human therapy, the high frequency and severity of certain injuries in race horses puts animal medicine at the forefront of this novel recovery process. Pets that are partners can serve as appropriate clinical models that mimic human diseases.
6) Ligament and tendon repair:
Autologous stem cell-based treatment for ligament injury, tendon injury, osteoarthritis, osteochondrosis, and sub-chondral bone cysts have been commercially available from veterinarians since 2003 in the United States and since 2006 in the United Kingdom.
Race horses are more prone to injury with tendons and muscles. The most common treatment has failed to restore the horse to full function. Natural healing, the most effective treatment, leads to the formation of red fibers that reduce flexibility and full joint movement. The use of embryonic stem cells has also been used in muscle repair. Embryonic stem cells were it has been shown that there is a better rate of tender survival and better migration skills to reach all areas injured muscles. The overall quality of the repair was also high, with better tender construction and collagen.
7) Joint-Repair:
Osteoarthritis is a major cause of joint pain in animals and humans. Horses and dogs are very common affected by arthritis. Cartilage regeneration is very limited and there are no current treatments, however instead look to reduce the symptoms associated with the damage. Different types of mesenchymal stem cells and other additives are still being considered for advanced cell type and long-term treatment
Stem Cell Therapies in Phase III Clinical Trials:
Cardiac repair:
The use of a patient's bone marrow, hematopoietic stem cells and mesenchymal stem cells (MSCs), in repairing heart tissue, can be confusing because these cells do not usually contribute to the desired cardiovascular system. There is some detail on lowering blood pressure in the body to improve cardiovascular function myocardial infarction15.
Also, research needs to determine the potential tropic effect of stem cell secretions or cytokines released at the site of injury and the degree of cardio correction that may be clinically appropriate16.
It is very clear that in the required ventricular remodelling, cell types work much better large volume filling will need to replace the most damaged area for heart.
Neurological applications:
Studies involving spinal blood for vascular indicators have been promoted due to data preceding the apparent formation of neurons in-vitro17. but less evidence of their variable differentiation into active neurons or glial cells in-vivo. Side blood cells (CD34 +) sent to the female vein have been used in safety studies for chronic spinal cord injury without side effects but with little evidence of follow-up function.
STEM CELL TRANSPLANT SIDE EFFECTS:
Because chemotherapy can cause nausea and severe vomiting, doctors often prescribe anti-nausea medication at the same time as chemo to try to prevent it.
3) Infection:
About the first six weeks after implantation, until the new stem cells begin to form white blood cells (engraftment), you can easily get serious infections. Bacterial infections are very common during this time, but infections that are controlled by your immune system can also work. Fungal infections can also be a problem.
4) Bleeding and transfusions:
After a transplant, you are at risk of bleeding because corrective action kills your body's ability to produce platelets.
MARKETED PRODUCTS:
Most SC treatments on the market and in development use MSCs. At least three osteobiologic products, Osteocel (NuVasive), Trinity (Orthofix) and LiquidGen (Skye Orthbiologics), use MSCs as part of the allograft matrix and are believed to promote osteogenesis and reduce inflammation SC grafts is the fastest growing bone graft market18.
REFERENCE:
1. Fact Sheet. what are Stem Cells Last updated: July 2010 [www.stemcellcentre.edu.au].
2. Kalra K and Tomar PC, Stem Cell: Basics, Classification and Applications. American Journal of Phytomedicine and Clinical Therapeutics, AJPC.2014; 2(7)91-930
3. International Society for Stem Cell Research, stem cell fact 2011[ http://www.isscr.org].
4. Friedenstein AJ, Piatetzky S II, Petrakova KV. Osteogenesis in transplants of bone marrow cells. J Embryol Exp Morphol 1966; 16:381–90.
5. Jesse.K. Biehi, B.S.and Brenda Russell. Introduction to Stem Cell”, J. Cardiovasc.Nurs.2009; 24(2):1-10.
6. Stem cell basic Last modified on April 08, 2015 [http://stemcells.nih.gov/info/].
7. S. Avasthi, R. N. Srivas tava, A. Singh and M. Srivastava ―Stem cells: Past, Present, Future –a review article‖, Internaet J. Med. Update, 2008, 3(1):22-30
8. juanv.files.wordpress.com/.../stemcells2-gif.
9. Schöler HR, The Potential of Stem Cells: An Inventory". In Nikolaus Knoepffler, Dagmar Schipanski, and Stefan Lorenz Sorgner. Humanbiotechnology as Social Challenge. Ashgate Publishing, Ltd. 2011; 1:380.
10. Hans R. The potential of stem cells: An inventory. Human biotechnology as Social Challenge, England, Ashgate Publishing, Ltd.2007; 28
11. http://www.medicalnewstoday.com/info/stem_cell/).
12. I Singec, R. Jandial, A. Crain, G. Nikkhahand E.Y. Snyder, "The leading edge of stemcell therapeutics". Annu. Rev. Med. 58, 2007, 313–328.
13. C.R. Freed, P.E. Greene, R. E Breeze, W-Y Tsai, W. DuMoucel, R. Kao, S. Dillon, H. Winfield, S. Culver, J.Q. Trojanowski, D. Eidelberg and S. Fahn, “Transplantation of embryonic dopamine neurons for severe parkinson’s disease” New England J. of. Med., 344, 2000, 710-719.
14. WWW.Wikipedia.com accessed on: 25 Dec 2018).
15. Copeland N, Harris D, Gaballa MA: Human umbilical cord blood stem cells, myocardial infarction and stroke. Clin Med 2009, 9(4):342-345.
16. Wollert KC, Drexler H: Cell therapy for the treatment of coronary heart disease: a critical appraisal. Nat Rev Cardiol 2010, 7(4):204-215.
17. Ali H, Bahbahani H: Umbilical cord blood stem cells – potential therapeutic tool for neural injuries and disorders. Acta Neurobiol Exp (Wars) 2010, 70(3):316-324.
18. Basharut A. Syed and James B. Evans; Visiongain. Stem Cell Technologies: World Market Outlook 2012–2022 doi: 10.1038/nrd3953. Visiongain (2012).
Received on 16.03.2021 Accepted on 19.12.2021
Accepted on 22.06.2022 ©A&V Publications all right reserved
Research J. Topical and Cosmetic Sci. 2022; 13(1):21-26.
DOI: 10.52711/2321-5844.2022.00004