1. INTRODUCTION:

Hair loss is a complex problem which is often distressing and can have a significant effect on individuals’ quality of life. It is a chronic and progressive condition affecting at least 50% of women by age 50. The most common cause of hair loss, androgenetic alopecia (female pattern hair loss), affects at least 40% of women, and will progress without treatment. Hair loss often affects self-esteem and personal attractiveness, potentially leading to depression and other negative effects. Hair loss may begin in women as early as their teens or 20s and increases significantly with age.

Hair is a fast growing element, which needs a healthy body and a balanced supply of nutrients to grow correctly. Any hormonal, blood, metabolic, or psychological changes as well as an imbalanced diet may exert a negative influence on the lifespan of hair; this is why any change affecting hair acts as a signal for general health of a person. Hair growth occurs through different stages within which the hair follicle experiences first strong developments, followed by metabolic rest periods as well as quite regressive or shedding processes. Hair loss is based on various biochemical mechanisms including inflammation and oxidative stress, androgenic metabolism, reduction of microcirculation, and alteration of the cross-talk between the matrix cells and dermal papilla (Braun N et al., 2020). Despite being part of the natural aging process, which also affects the scalp, it can negatively affect the quality of life of each individual, with situations that can lead to a state of psychological distress and demoralization. It is important to understand whether hair loss falls within the normal physiological process, or otherwise, if it is an abnormal complication caused, for example, by problems related to the scalp or hair bulb. The classification of all forms of hair loss, baldness and alopecia, is always complex and reductive, since they are often the expression of multiple factors that contribute to the onset of these pathologies. In women, androgenetic alopecia is one of the main causes, but the incidence is extremely minor, due to the reduced presence of androgens. Aggressive chemical and physical treatments that are applied to the women’s scalp are responsible for the weakening of the hair, often resulting in hair loss. Conversely, the common causes that can be simultaneously associated in man and woman thinning hair are different, such as the so-called seasonal hair loss, in spring and autumn. Even this phenomenon, although temporary, should be kept under control, in order to not compromise any already critical situations. There is a link between hair loss and diet, since hair is made by proteins for 65-95%; among other components are lipids, pigments, oligo elements and water. It is therefore necessary to intake specific nutrients and micronutrients. However, mistakes are often made and they can lead to an unbalanced diet. Among these common mistakes we can find low or no consumption of fruit and vegetables as well as following very restrictive diets in general. Due to this fact, proteins are sometimes missing, especially those containing Sulphur amino acids, such as methionine and cysteine. The lack of such amino acids can lead to hair brittleness, decreased caliper and dry hair. A diet containing low amino acids, minerals and vitamins may lead to severe hair loss, which can be reversed if a balanced intake of nutritional elements takes place shortly after (Rinaldi et al., 2003).

Chicken egg shell and membrane are byproducts of eggs. The egg shell membranes are true source of valuable bioactive materials, including collagen that has many applications in medical, health and cosmetics. Eggshell membranes are an abundant raw material that are a novel source for naturally occurring bioactive compounds such as glucosamine (Picard et al., 1973) chondroitin sulfate (Baker et al., 1962), hyaluronic acid (Long FD et al., 2005), fibrous proteins such as collagen Type I (Wong M et al., 1984) and sulfur-rich proteins (Tsai et al., 2006) and other components including lysozyme, ovotransferrin, ovocalixin and desmosine and Isodesmosin. Glycosaminoglycan and proteins essential for maintaining healthy joint and connective tissues

Collagen Type (I, V, X) is the most important protein produced by the human body, it is mainly formed by the amino acid glycine (33%), Proline and hydroxyproline (22%) (primary structure) in a triplex helix which is formed by three α chains (Sorushanova A. et al., 2019). Elastin is a protein critical to skin, cardiovascular, cartilage and spinal column health. As its name suggests, elastin gives tissue the elastic tension and ability to resume their shape after stretching. Desmosine and Isodesmosine - two little known amino acids that is responsible for elastin’s elastic and rubbery properties. Chondroitin sulfate is a sulfated glycosaminoglycan (GAG) composed of a chain of alternating sugars (N-acetylgalactosamine and glucuronic acid). It is usually found attached to proteins as part of a proteoglycan. Chondroitin sulfate is an important structural component of cartilage and provides much of its resistance to compression. The eggshell membrane contains a number of bioactive components that may impact the physiology and presentation of the hair. For example, the eggshell membrane is rich in glycosaminoglycan’s (GAGs). GAGs are highly negative charged polysaccharides that appear to influence bio mineralization. GAGs are complex molecules and can be part of larger molecules.

Ellagic acid is a natural chemical in certain foods. It is also classified as a phenolic compound. Sometimes, it is rather referred to as a polyphenol antioxidant. Ellagic acid, also known as 2,3,7,8-tetrahydroxy (1) benzopyrano(5,4,3-CDE) (1) benzopyran-5,10-dione, is a well-known molecule belonging to the group of polyphenols. Ellagic acid can also be used in antidandruff shampoo preparations. Ellagic acid-containing formulation has been reported for treating graying of the hair.

Emblica officinalis (Indian gooseberry or Sabbery), more commonly known as Amla. The dried fruit, which improves hair hygiene, has long been utilized as an important ingredient of shampoo and hair oil. Thus, the Indian gooseberry is used as a hair tonic in traditional recipes for enriching hair growth and pigmentation. It contains a minimum of 10% ß-glucogallin and 50% gallates. ß-glucogallin is a powerful antioxidant molecule and rejuvenator which prevents hair loss (Yu et al., 2017).

Biotin (also known as vitamin B7 or vitamin H) is a water-soluble vitamin that serves as an essential cofactor for carboxylase enzymes in multiple metabolic pathways. The use of these agents for hair loss is based on the observation (Patel et al., 2017). Curcumin, the active ingredient of turmeric, has been used for centuries as an anti-inflammatory agent. Curcumin counters the overproduction of a hormone known as DHT (dihydrotestosterone). It is a hormone known to cause hair loss by preventing new hair growing from your follicles. It is a powerful antioxidant which reduces hair loss, thinning of hair, reduces hair loss through breakage (Pumthong et al., 2013).

Murraya Koenigii is long known for its properties to strengthen hair roots. It has Vitamin A, Vitamin B, Vitamin C, Vitamin B2, Calcium and iron in plenty (Singh et al., 2015). Promotes hair growth, prevents hair thinning and hair loss.

Intent of this study is to evaluate whether oral supplementation with a specific eggshell membrane that contains a number of bioactive compounds that improves appearance and health of hair. The aim of this study is to evaluate the impacts of Trichovitals ^TM composed of Muttaijow Complex ® on the appearances and health of hair in healthy young aged adults.

2. MATERIALS AND METHODS:

The following Composition is endorsed in Central FSSAI License by Microcore Research Laboratories India Private Limited and the brand is registered under TM Act, 1999 Certificate of Registration of Trade Mark, Section 23(2), Rule 56(1) Trichovitals® and the Statement of the Ingredient is as followed by,

Statement of Ingredients and Additives (For Food Categories 13.6)

Ingredients:

Merits of ingredients:

Natural egg shell membrane (Muttaijow Complex^®):

Muttaijow Complex® is excellent source of Collagen (I, III, V, X, Elastin (Desmosin + Isodesmosin) formulated at 455mg/capsules. In general Collagen holds dermal layer and hair follicles together which in turn keeps hair strong and healthy when collagen production reduces due to ageing, hair follicles becomes brittle, while the dermal layer gets dried up, resulting in hair fall. Collagen begins to decline from the age of 25years. Collagen supports strong healthy skin hair.

Ellagic Acid:

Ellagic Acid (90%) it is an extract from pomegranate fruit and used at 5mg/capsules. Pomegranate has the highest concentration of ellagic acid. Ellagic acid-containing formulation has been reported for treating graying of the hair. Ellagic acid has excellent hair growth promoting activity by enlarging the follicular size and prolongation of anagen (growing) phase.

Emblica officinalis- dried fruit extract:

Emblica officinalis, dried fruit extract from Amla fruit used at strength of 5mg/capsule. Amla fruit extract is a powerful antioxidant, rejuvenator which prevents hair loss. Amla, a vitamin-rich fruit which is beneficial for hair in numerous ways. It helps in reducing dandruff by removing the flakiness and greasiness of the scalp. It also nourishes the hair and hair follicles that helps in the reduction of hair fall. It helps in balancing the sebum content of your scalp. They shrink the hair surface as well as tighten and make the hair stronger and healthier by coating the hair cuticle.

Biotin:

Biotin (a water soluble vitamin) used at 25mcg/ capsule. Biotin, also known as vitamin B7, stimulates keratin production in hair and improves the infrastructure of keratin, a basic protein that makes up hair. It can improve hair health-including volume, scalp coverage and can increase the rate of follicle growth. Biotin can fight inflammation, a leading cause of hair loss. This may suggest that biotin can be used as a preventative measure to fight hair loss.

Curcuma longa extract:

Curcuma longa extract is used at the strength of 5 mg/Capsules. Tetrahydrocurcumin is a beta-diketone that is curcumin in which both of the double bonds have been reduced to single bonds. curcumin's proposed antioxidant properties could help hair to grow longer and stronger. Curcumin is both gentle and invigorating and makes for a wonderful exfoliator cleanser for the hair and scalp. It could help to lower the levels of hormone DHT, which can be responsible for some types of hair loss. Curcumin Reduces hair loss, thinning of hair, reduces hair loss through breakage.

Murraya Koenigii:

Murraya Koenigii (An extract from curry leaf) used at concentration of 30mg/Capsule. Curry leaves are packed with vital nutrients and used to ease a variety of ailments. They are useful for hair because they are high in beta-carotene and protein content, which are instrumental in preventing hair loss and hair thinning. Proteins are necessary for hair growth because hair is made of protein and is also vital for hair development. The amino acid content that strengthens hair fiber is also high in curry leaves. Murraya Strengthens hair roots, promotes hair growth, prevents hair thinning and hair loss.

2.1 Food supplement under investigation:

The dietary supplement used in this study was Muttaijow Complex® consisting of egg membranes separated from eggshells by a patented process by Microcore Research Labs India Pvt ltd www.microcorereesarch.com. Muttaijow Complex® is already clinically studied by Microcore for the health maintenance of the joints and connective tissues www.jointcore.us

a) Clinical Trial Registry of India Ref No: - CTRI/2021/08/035335

b) PM ethics committee Ref No: -JOINTCORE/031/21

c) AYUSH ethics committee Protocol Ref No: - JOINTCORE/031/21

Samples of Muttaijow Complex ® were later retrieved for composition analyses followed by AOAC official method, 2019. Compositional analysis of eggshell membranes has identified a high content of protein (collagen types I-V-X, elastin, keratin) (Wong et al., 1084; Arias et al., 1991) and moderate quantities of GAGs (glucosamine, chondroitin sulfate, HA) (Picard, Paul-Gardais, and Vedel, 1973). We have confirmed the presence of the major components collagen, HA, Glycoaminoglycans in Muttaijow Complex^® composition.

2.2. Pilot study:

2.2.1 Selection of participants:

The study was conducted in Tamil Nadu, Southern part of India at Erode district. The study enrolled a group of 50 young aged healthy volunteers (12- Male; 37- Female). Volunteers were randomized in double- blind fashion to receive the oral supplement (Trichovitals^®) or placebo (Maltodextrin). 50% of volunteers were in the age range of 19-21 years; 35% were 21-25 years; and 15% were 25-28 years old (Table 1).

2.2.2 Study design:

Self-assessment questionnaire:

Participants took 500 mg of Trichovitals ^®daily for 90 days. Products were taken orally at home according to the instructions given by the investigator. The study consisted of a baseline and a follow-up visit at the end of 90 days’ treatment. Participants completed a self-assessment questionnaire considering six parameters as follows Overall hair volume, Scalp coverage, Thickness of hair body, Softness of hair body, Hair shine regarding their experience with the regimen at 90 days of treatment.

Table 1: Baseline demographic details of the study participants

Parameters	Trichovitals^® (mean±SD)	Placebo (mean±SD)
Age (years)	25.08 + 8.75	26.33 + 8.94
Gender Male (n) Female (n)	5 19	7 18
BMI (Kg/m²)	25.80 + 2.38	26.73 + 1.54

Flow Diagram

Figure 1: Flow diagram of the study.

Pull Test:

Pull test is used to assess the resistance of hair stem to traction. This is also known as the ‘traction test’ or ‘Sabouraud’s sign’ or the ‘pull-out sign.’ Approximately 20-60 hairs are grasped between the thumb, index and middle fingers from the base of the hairs near the scalp and firmly, but not forcefully, tugged away from the scalp (Dhurat et al., 2009). Volunteers were subjected to pull test during baseline visit and after 90 days of Trichovitals ^® consumption to study the effect of Trichovitals ^®in increasing hair strength. Results of pull test were reported according to the following score system:

0 = little or very little resistance

1 = moderate resistance

2 = high resistance

Hair count measurement

Physician selected an approximately 4cm² area of scalp along the frontalis bone at the junction of the frontal and lateral hairlines. This location was identified for further assessments using a three-point location noted on each volunteer according to measurements taken from medial canthus, lateral canthus, and preauricular skin pit to the hairline junction. Following the baseline visit, volunteers returned for evaluation after 90±7 days and the identified location in the scalp was considered for hair count measurement. The mean number of Terminal and vellus hairs of volunteers were measured to observe the effect of Trichovitals^®in increasing the hair volume.

Wash test:

Wash test is used to assess the number of hairs lost when hair was washed. In the wash test, the patient refrains from shampooing for 5 days and then he/she shampoos and rinses the hair in the basin with the hole covered by gauze. The hairs remaining in the water and the gauze at the end of the wash process are collected and counted by the examiners (average of subjective values reported by all subjects at each wash time) (Chamberlain et al., 2003). Results of Wash test procedure performed during baseline visit and after 90 days of Trichovitals^® consumption was compared to observe the effectiveness of Trichovitals^® in reducing hair loss.

60-Second Hair count:

60-second hair count is a simple, practical, and reliable tool for the assessment of hair shedding. The volunteers were issued identical combs to be used throughout the study. The teeth of the comb, which was 15cm long, were separated by 1mm on one half of the comb and by 2mm on the other half. During the baseline visit, volunteers were instructed to comb their hair for 60 seconds before shampooing in the morning, starting at the vertex and combing forward by using the comb provided for the study. The hair was combed over a towel or pillowcase of contrasting color so that any shed hairs could be adequately visualized. The volunteers then collected the shed hairs and recorded their number. This “comb-and-count” technique was repeated on 3 consecutive days. The volunteers returned all hairs and data sheets to the investigators. After regular consumption of Trichovitals^®for 90 days, volunteers were instructed to repeat the entire procedure exactly as before and collected shed hairs were counted.

3. RESULT AND DISCUSSION:

3.1 Compositional analysis of 500 mg of Microcore Muttaijow Complex^®

Amino acid composition of Natural Egg Membrane Concentrate for Lysine (2.98%), Tryptophan (2.71%), Leucine (3.96%), Aspartic acid (7.05%), Proline (8.25%), Isoleucine (2.12%), Threonine (4.67%), Glycine (4.01%), Histidine (2.91%), Arginine (5.36%), Tyrosine (1.34%), Glutamic acid (8.25%), Cysteine (6.14%), Alanine (2.05%), Methionine (2.85%), Valine (5.15%), Phenylalanine (1.49%), Serine (4.3) were shown in the (Table 2). Constituents of Natural Egg Membrane Concentrate resulted 96% protein, 17% Collagen, 27% Elastin (16.2% Desmosine, and 10.8% Iso desmosine), 28.3% Chondroitin Sulphate, 20.5% Glycosaminoglycan and 5% Hyaluronic acid was shown in the (Table 3).

Table 2: Typical Amino acid composition of Natural egg shell membrane (Muttaijow Complex®)

S. No	Aminoacid	% Muttaijow Complex®
1	Lysine	2.98
2	Tryptophan	2.71
3	Leucine	3.96
4	Aspartic acid	7.05
5	Proline	8.25
6	Isoleucine	2.12
7	Threonine	4.67
8	Glycine	4.01
9	Histidine	2.91
10	Arginine	5.36
11	Tyrosine	1.34
12	Glutamic acid	8.25
13	Cysteine	6.14
14	Alanine	2.05
15	Methionine	2.85
16	Valine	5.15
17	Phenylalanine	1.49
18	Serine	4.3

Table 3: Typical constituents of Natural egg shell membrane (Muttaijow Complex®)

S.No	Sample	Muttaijow Complex® (%)
1	Protein	96
2	Collagen (Collagen Type (I,III,V and X)	17
3	Elastin	27
4	Desmosine	16.2
5	Isodesmosine	10.8
6	Chondroitin sulfate	28.83
7	Glycosaminoglycan	20.15
8	Hyaluronic acid	5

3.2 Results of test performed:

3.2.1 Self- assessment questionnaire:

Self-assessment questionnaire responses were analyzed, and results are shown in (Table 4). Volunteers were separated into Trichovitals^® treated group (N=24) and Placebo (N=25). After continuous intake of Trichovitals ^® for 90 days, volunteers were given self-assessment questionnaire to observe the changes in their hair conditions. Self-assessment questionnaire average scores of both Trichovitals^®treated volunteers and placebo were compared. Overall hair volume, Scalp coverage, hair body thickness, shinning of hair, Hair body softness was increased for Trichovitals^® treated volunteers in comparison to placebo group. There was a significant difference in all six parameters during the baseline visit and after 90 days of Trichovitals^®consumption.

Table 4: Self-assessment questionnaire

Sl. No.	Questionnaire	Trichovitals^®(%) (N=24)				Placebo (%) (N=25)
		Day 0		Week 12		Day 0		Week 12
1	In general, Overall Hair Volume:
	Excellent	0		10		0		0
	Very good	2		50		1		0
	Good	20		38		19		14
	Fair	65		2		67		70
	Poor	13		0		13		16
2	Hair Shine
	YES, Increased a lot		0		42		0	1
	YES, Increased a little		14		40		17	13
	NO, not increased		96		18		83	86
3	Scalp Coverage
	Yes, High		16		86		9	5
	No, Low		84		14		91	95
4	Thickness of Hair Body
	High		4		64		5	0
	Medium		20		23		35	27
	Low		76		13		60	73
5	Softness of Hair Body
	Greatly increased		4		40		6	0
	Moderately increased		9		40		8	0
	Slightly increased		12		20		9	0
	Greatly decreased		48		0		56	62
	Moderately decreased		25		0		16	19
	Slightly decreased		2		0		5	19
6	Number of hair lost on an average day
	Greatly increased		76		0		68	78
	Moderately increased		22		0		32	18
	Slightly increased		2		0		10	4
	Greatly decreased		0		69		0	0
	Moderately decreased		0		27		0	0
	Slightly decreased		0		4		0	2

3.2.1 Pull Test:

Trichovitals ^® treated volunteers (N=24) and Placebo treated volunteers (N=25) both group were subjected to pull test during their baseline visit and after 90 days of treatment, the results of pull test are recorded (Table 5). Scores of resistance to traction increased from 0.9 to 1.4, whereas placebo group of volunteers experienced low resistance to traction at day 90 (0.5) compared to scores of resistance (0.6) during baseline visit (Figure 2). Trichovitals ^® Increase in score of resistance to traction of treated volunteers, indicates that Trichovitals ^® consumption increases hair strength.

Table 5: Resistance to traction scores

Time of study	Resistance to traction scores, mean
	Treated volunteers(N=24)	Placebo (N=25)
Day 0	0.9	0.6
Day 90	1.4	0.5

Figure 2. Comparison of Pull test scores3.3 Pilot study-II:

3.3.1 Measurement of hair count:

Trichovitals^® consumption for 90 days showed a great improvement in hair count for treated volunteers (N=24) than placebo (N=25) group of volunteers. Visual improvement of hair count was observed during baseline visit and at end of 90 days after treating with Trichovitals^®. At baseline visit, mean number of terminal hair count for placebo group was 275 and remained at 252 after 90 days, whereas mean number of terminal hair count of Trichovitals^® treated group were increased from 292 to 542 at end of 90 days of treatment (Table 6). The mean number of vellus hairs among placebo-treated volunteers was 52 at baseline and 63 after 90 days (Figure 3). The mean number of vellus hairs among Trichovitals^® treated volunteers was 49 at baseline and 45 after 90 days. Trichovitals^®treated volunteers expressed overwhelming responses regarding improvement in their hair conditions.

Table 6: Changes in number of terminal and vellus hairs

Type of hair	Treated volunteers (N=24)		Placebo (N=25)
	Day 0	Day 90	Day 0	Day 90
Terminal hair	292	542	275	252
vellus hair	49	45	52	63

Figure 3. Measurement of hair count

3.3.2 Wash test:

Volunteers consuming Trichovitals^®for 90 days (N=24) and placebo group of volunteers (N=25) were advised to collect hairs after hair wash. Hair loss, assessed both in an objective and subjective way by counting hairs found in the gauze that covers the basin at the end of each washing. Average number of hair lost in wash test conducted during baseline visit and at end of treatment (90 days) was recorded (Table 7). Treated volunteers showed significant changes in their hair loss count from 375 during their baseline visit to 257 at the end of 90 days after their treatment, whereas placebo group of volunteers moreover had no change in their hair loss count from 282 at day 0 to 297 at day 90 (Figure 4). Treated volunteers was overwhelmed by the effect of Trichovitals^® in reduction of hair loss.

Table 7: Wash test hair lost count

Study group	Number of hair lost, mean
Study group	Day 0	Day 90
Treated volunteers (N=24)	375	257
Placebo (N=25)	282	297

Figure 4. Comparison of wash test

3.3.3 60-second hair count test:

60s hair count is easy-to-use tool for quantifying and tracking hair loss. Volunteers consuming Trichovitals ^®(N=24) and placebo group of volunteers (N=25) both willingly performed the hair counts so that they can gain some control over a condition in which they feel that they have no control. Average count of hair shed in 3 consecutive days during combing at beginning of the study (day 0) and at the end of the study (day 90) was collected (Table 8). Trichovitals^® consumption had a great effect in reducing hair lost count to (37.28) at end of the study from (50.71) during baseline visit. Placebo group of volunteers had no significant changes in their hair loss count in comparison to treated group (Figure 5).

Table 8: 60 second hair count test

Study group	Number of hair lost, mean
	Baseline visit			End of study
	Day 1	Day 2	Day 3	Day 1	Day 2	Day 3
Treated volunteers (N=24)	13.28	16.28	21.14	10	11.14	16.14
Placebo (N=25)	19	22.66	26.66	21.33	27.66	31.33

Figure 5. 60 second hair count test

4. CONCLUSIONS:

Trichovitals^®consumption on a regular basis had a significant change in several hair parameters. The following results were observed from the Pilot studies. In this pilot study, self-assessment questionnaire is investigated to check the effect of Trichovitals^®on hair. Volunteers consumed Trichovitals^®on daily basis for 90 days were greatly satisfied with their hair conditions. High scores were received from treated volunteers in comparison to placebo group of volunteers for all parameters (Overall hair volume, Scalp coverage, Thickness of hair body, Softness of hair body, Hair shine) that was taken into consideration. In pull test, resistance to traction scores was high in Trichovitals^® treated volunteers in comparison to placebo group, this confirms that Trichovitals^® consumption has a significant effect on improving hair strength. Measurement of hair count was performed to check the efficacy of Trichovitals^® in increasing the hair count and volume. Terminal hair count has increased in great scale after consuming Trichovitals^® for a period of 90 days. In general shedding of wet hair is high than dry hair, wash test was performed to notice the reduction in hair lost count during hair wash. Trichovitals^® treated volunteers had notable reduction in hair lost count during washing than placebo group. 60-second hair count a practical, and objective tool for monitoring conditions associated with hair shedding showed that Trichovitals^® consumption on daily basis had a great impact on reducing the hair lost during combing. In conclusion, Trichovitals^® treated volunteers experienced improvement in overall hair parameters considered for study and was overwhelmed by the results of Trichovitals^® on their hair conditions.

5. REFERENCES:

1. Braun N, Heinrich U. What Can Complex Dietary Supplements Do for Hair Loss and How Can It Be Validly Measured—A Review. Appl. Sci. 2020; 10, 4996. doi:10.3390/app10144996

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3. Picard J, Gardais AP, Vedel M. Sulfated glycoproteins from egg shell membranes and hen oviduct. Isolation and characterization of sulfated glycopeptides. Biochim. Biophys. Actavol. 1973; 320, pp. 427-441.

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5. Long FD, Adams RG, DeVore DP. Preparation of hyaluronic acid from eggshell membrane. USA Patent. 2005; 6946, 551.

6. Wong M, Hendrix MJC, Von der MK. Collagen in the egg shell membranes of the hen. Dev. Biol. 1984; vol-104 pp. 28-36.

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8. Sorushanova A et al. The collagen superfamily: From biosynthesis to advanced biomaterial development. Adv. Mater. 2019; 31, 1801651.

9. Yu JY et al. Preclinical and Clinical Studies Demonstrate That the Proprietary Herbal Extract DA-5512 Effectively Stimulates Hair Growth and Promotes Hair Health. Evidence-Based Complementary and Alternative Medicine. 2017; Article ID 4395638, 11 pages. https://doi.org/10.1155/2017/4395638

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Received on 27.04.2022 Accepted on 28.05.2022

Research J. Topical and Cosmetic Sci. 2022; 13(1):1-8.

DOI: 10.52711/2321-5844.2022.00001

S. No	Ingredients (Name of the Ingredients)	Schedule under Nutraceuticals Regulations in which the ingredients is permitted for use/ Product Approval Copy	Qty (mg)	Purity Criteria adopted
1	Natural Egg Shell Membrane	02/Std/PA/FSSAI/2021 and 02/Std/PA/FSSAI/2021 (part-1) Approval for Non-specific Food and Food Ingredients) Regulations, 2017	455	Yes
2	Curcuma longa extract (Taetra Hydro Curcumin)	Schedule -VI, PART-B(56) Functional food 97	5	Yes
3	Ellagic acid (an extract from Pomegranate red pigment)	Schedule -VI, PART-B (62)	5	Yes
4	Emblica officinalis- dried fruit extract(spray dried powders) Indian goose berry/Amla	Schedule VI,PART-B (No- 63)	5	Yes
5	Murraya Koenigii(L) Spreng /M exotica Linn (Kamini) (Leaf)	Schedule IV, (No-246)	30	Yes
6	Biotin	F.NoStds/Nutra (DCGI)/FSSAI-2017	25mcg	Yes