Effects of Oral Supplementation of Muttaijow complexTM on Human Skin, Hair and Nail Physiology

 

 

Chandramohan Marimuthu*, Jayaganesh Murugan, Srisunmathi Ravichandran, Sruthi Sukumar

Microcore Research Laboratories India Pvt Ltd., 9th km, 30 Feet Road, 204 - A Poondurai Main Road, Checkmedu, Erode - 638115, Tamil Nadu.

*Corresponding Author E-mail: info@microcoreresearch.com

 

ABSTRACT:

In this study, Microcore’s oral supplements of Muttaijow ComplexTM obtained from the egg shell that contains naturally occurring collagen Type (I, V, X), Elastin (Desmosine and isodesmosine), Hyaluronic acid, Glucosamine, Chondroitin, Glycosaminoglycans or Muco-polysaccharides, Membrane bound protein molecules, and Calcium are used in a group of volunteers. This study leads to evaluate the impacts of a nutritional intervention as compared to control group (without oral supplements intake) on the appearances of hair, skin, and nails in healthy middle-aged adults. Two pilot studies were run to assess Muttaijow ComplexTM on skin parameter. 1. Self-assessment questionnaire intended to perform an assessment on skin, hair, and nail health after 12 weeks of treatment for a group of 25 female volunteers 2. Evaluated the efficacy of Muttaijow ComplexTM effect of 5-week treatment on hydration of skin by corneometry, on elasticity with the cutometer, and on pigmentation with themexameteron for a group of 7 female volunteers, Microscopic studies of hair strength and nail growth. In study 1-Participants were predominantly satisfied with the effects obtained on general face (100% volunteers satisfied) and body (94% volunteers satisfied) skin condition and skin properties (100% volunteers satisfied with facial skin softness, 95% with facial skin hydration, 83% of facial skin brightness and 89% with body skin hydration) and partly with effects on hair (65-70%volunteers satisfied) and nail appearance (70% volunteers satisfied) and improvement in brittle nails. In this open-label 5 weeks study, Oral Microcore’s Muttaijow ComplexTM ingestion was associated with a significant improvement in skin appearance such as increase skin hydration and skin elasticity, reduces skin pigmentation after 5 weeks treatment and facial skin tone, with significant impact on hair thickness, reduces split ends, hair loss, hair breakage and improvement in hair growth after 5 weeks treatment, increase of 12% nail growth rate and a decrease of 42% brittle nails after 6 weeks. Microcore’s Muttaijow ComplexTM achieved significant change improvement in skin, hair and nail appearance (thickness/density) than control group (without oral supplements intake) by week 12 of the study. From the result analysis, Microcore’s Muttaijow ComplexTM is deemed to be one of the most effective anti-aging products in cosmetic sector and overall physical appearance. Oral supplementation of 500mg/day of Microcore’s Muttaijow ComplexTM for 12 weeks is associated with significant improvement in the appearance of facial skin, hair and nail. Increases in skin hydration and skin elasticity with reduced pigmentation on skin were observed after 5 weeks treatment, which is a very important marker for skin aging. Also, significant impact on hair thickness, reduces split ends, hair loss, hair breakage and improvement in hair growth and healthy development of nails after 5 weeks treatment.

 

KEYWORDS: Collagen, Eggshell, Oral supplement, Pilot study, Skin, Hair, Nail.

 

 

 

 

 

1. INTRODUCTION:

In the human body, skin is the largest organ and is involved in several important functions. The two main layers that make up the skin are the outer layer, or epidermis, and the inner layer, or dermis. The epidermis is composed of a stratified squamous epithelium that overlies a basal layer composed of columnar cells arranged perpendicularly (proliferating and differentiated keratinocytes). The dermis is composed of connective tissue consisting of diverse extracellular matrix components, including collagen and elastin fibers and glycosaminoglycans (GAGs), Shaw G et al. (2017), which are synthesized by dermal fibroblasts. Underlying these layers is subcutaneous fat tissue.

 

In the effort to halt or slow aging, consumers often turn to interventions that may include cosmetic dermatology, over-the-counter products and dietary supplements. It has been estimated that in 2017, sales of products and services in this category hit $5.16 billion dollars.1 According to Nielson, in 2018, Americans spent $12 billion dollars on beauty and personal care products, with hair, skin, and nails falling under this category. (Bagchi D et al., 2002). While there are many products and services available to address skin and hair issues, the efficacy of many of these products and services has been questioned. Therefore, due to the extensive cost to the consumer and the need for claim substantiation of such products, evaluation of efficacy is warranted.

 

It is known that the appearance of the hair, skin, and nails changes as part of the aging process. This is due to normal intrinsic and extrinsic aging as well as to environment exposures such as UV sunlight. For example, the collagen density of the dermis decreases with age, which leads to a reduction in dermal thickness. Similar changes also impact hair and hair appearance (shine, thickness, fullness, etc), and more specifically, molecular alterations lead to structural modifications of the hair fiber, decrease in melanin production, and lengthening of the telogen phase of the hair cycle, and thus altered hair appearance and health. The appearance of the nails also can change due to aging, including alterations in nail thickness, contour, surface, and color. Kompel AJ et al. (2019) Therefore, approaches to offset or even reverse these changes are desirable to consumers.

 

Young skin is firm, smooth, and of radiant appearance; nevertheless, profound changes occur in the structure of the dermis and epidermis over time through processes of intrinsic (chronological aging) and extrinsic (photo aging) aging (Helfrich, Sachs, and Voorhees, 2008).

 

Skin changes associated with natural aging are generally characterized by fine wrinkling and laxity, which can be worsened by chronic ultraviolet (UV) light exposure. Clinical signs of photo aging include dryness, deep furrows, irregular pigmentation, elastosis, and a leathery appearance (Helfrich, Sachs, and Voorhees, 2008). The dermis provides structure and support for the epidermis, as well as for the vasculature and appendages of the skin such as hair and nails. As a result, the structural integrity of the dermis is vital for the normal function and youthful appearance of the skin As the skin ages, the number of collagen and elastin fibers in the dermis decreases, and total skin thickness decreases (Helfrich, Sachs, and Voorhees,2008). Collagen and elastin fibers become sparse and increasingly disordered, leading to formation of wrinkles and sagging of the skin (Helfrich, Sachs, and Voorhees, 2008).

 

Eggshell membrane, an organic substance, is known to increase cellular activity, increase collagen production, slow skin aging.Chicken egg shell and membrane are byproducts of eggs. The egg shell membranes are true source of valuable bioactive materials, including collagen that has many applications in medical, health and cosmetics. Eggshell membranes are an abundant raw material that are a novel source for naturally occurring bioactive compounds such as glucosamine (Picard et al., 1973) chondroitin sulfate (Baker and Balch, 1962), hyaluronic acid (Long FD et al., 2005), fibrous proteins such as collagen Type I (Wong M et al., 1984) and sulfur-rich proteins (Tsai et al., 2006) and other components including lysozyme, ovotransferrin, ovocalixin and desmosine and isodesmosin. Glycosaminoglycan and proteins essential for maintaining healthy joint and connective tissues.

 

Collagen Type (1,V,X):

Is the most important protein produced by the human body, it is mainly formed by the amino acid glycine (33%), proline and hydroxyproline (22%) (primary structure) in a triplex helix which is formed by three α chains (Sorushanova A. et al., 2019). Elastin is a protein critical to skin, cardiovascular, cartilage and spinal column health. As its name suggests, elastin gives tissue the elastic tension and ability to resume their shape after stretching. desmosine and isodesmosine - two little known amino acids that is responsible for elastin’s elastic and rubbery properties.

 

Chondroitin sulfate:

Is a sulfated glycosaminoglycan (GAG) composed of a

chain of alternating sugars (N-acetylgalactosamine and glucuronic acid). It is usually found attached to proteins as part of a proteoglycan. Chondroitin sulfate is an important structural component of cartilage and provides much of its resistance to compression.

 

The eggshell membrane contains a number of bioactive

components that may impact the physiology and presentation of the hair, skin, or nails. For example, the eggshell membrane is rich in glycosaminoglycans (GAGs). GAGs are highly negative charged polysaccharides that appear to influence biomineralization. GAGs are complex molecules and can be part of larger molecules. One such example is proteoglycans, proteoglycans are glycosylated molecules where one or more specific glycosaminoglycan (GAG) and/or O- and N-linked oligosaccharides are attached to a core protein (Liu Z, Sun X, Cai C, He W, Zhang et al,. 2016). There are several types of GAGs which are typically sulfated. These include chondroitin sulfate/dermatan sulfate (CS/DS), keratan sulfate (KS), and heparan sulfate (HS)/heparin. There are four specific GAG types in the chicken eggshell, hyaluronan, keratan sulfate, chondroitin sulfate, and heparan sulfate. Hyaluronan is the most prevalent GAG in eggshell membranes.

 

Eggshell membrane contains hyaluronan, thus also contains the GAG hyaluronic acid (HA). HA production occurs in the skin and has been shown to stimulate internal production of HA with oral intake. Keratan sulfate (KS) is also a natural GAG occurring in the hydrolyzed eggshell membrane. (Oe M, Sakei S, Yoshida H, et al.2017) In the body, KS may influence wound healing, especially that of the skin. Chondroitin sulfate (CS), another GAG, has also been tested for its ability to impact skin health.

 

Oral supplementation of eggshell membrane has been linked with positive improvements in skin elasticity and appearance of the skin. It was the intent of this study to evaluate whether oral supplementation with a specific eggshell membrane that contains a number of bioactive compounds that improves appearance, health of hair, skin, and nails The aim of this study is to evaluate the impacts of a nutritional intervention on the appearances of hair, skin and nails in healthy middle-aged adults and to study the effectiveness of Muttaijow ComplexTM composed of egg membrane, on skin biophysical parameters, such as elasticity, pigmentation, and hydration, related to cutaneous aging.

 

2. MATERIALS AND METHODS:

2.1 Food supplment under investigation:

The dietary supplement used in this study was Muttaijow ComplexTM consisting of egg membranes separated from eggshells by a patented process. Samples of Muttaijow ComplexTM were later retrieved for composition analyses followed by (George W & Latimer JR, 2019) AOAC official method. Compositional analysis of eggshell membranes has identified a high content of protein (collagen types I-V-X, elastin, keratin) (Wong et al., 1084; Arias et al., 1991) and moderate quantities of GAGs (glucosamine, chondroitin sulfate, HA) (Picard, Paul-Gardais, and Vedel, 1973).We have confirmed the presence of the major components collagen, HA, Glycoaminoglycans in Muttaijow ComplexTM composition.

 

2.2. Pilot study level – I:

2.2.1 Selection of participants:

The study was conducted in Tamil Nadu Southern part of India at Erode district. The study enrolled a group of 25 adults, healthy volunteers of female. 40% of volunteers were in the age range of 30–45 years; 27% were 46–60 years; and 33% were over 60 years old.

 

2.2.2 Study design:

Participants took 500 mg of Microcore Muttaijow ComplexTM daily for 12 weeks. Products were taken orally at home according to the instructions given by the investigator. The study consisted of a baseline and a follow-up visit at the end of treatment. Participants completed a self-assessment questionnaire regarding their experience with the regimen at 12 weeks. The questionnaire comprised three sections intended to perform an assessment on skin, hair, and nail health. The first part consisted of nine questions related to skin health. The second part included seven questions related to hair health, and the final section comprised four questions about nail health. Each question has five possible answers with an assigned score: (70-100%) great improvement, (30-60%) mild improvement, (0%-10%) no change. Finally, we calculated the percentage of volunteers giving a specific answer (Asserin J, et al.,2015)

 

2.3 Pilot study – II:

2.3.1 Selection of participants:

The study was conducted in Tamil Nadu Southern part of India at Thiruchirapalli district. The study enrolled a group of 7 volunteers were in the age range of 25–60 years. Participants were treated daily over a 5-week period with oral supplementation of 500 mg of Microcore’s Muttaijow ComplexTM for skin, hair and nail testing parameters.

 

2.3.2 Study design:

Skin Parameter Testing:

Participants had the following assessments at each visit: skin hydration, skin pigmentation, and skin elasticity. Measurements were performed with the multifunctional skin analyzer MSD800 (Courage and Khazaka, Cologne, Germany), which allows measurement of skin parameters using different probes such as a corneometer, acutometer, and amexameter. The corneometer assesses skin surface hydration by electrical capacitance, which is the most common method used to determine the skin surface moisture (Proksch et al., 2014).

 

It measures the reactive capacitance of skin, using the

stratum corneum as a dielectric membrane. Measurements are arbitrarily expressed as indices of hydration (0–99), which increase with increasing skin hydration. A score less than 30 implies very dry skin; a score of 30–60 indicates dry skin; and a score over 60 implies sufficiently moisturized skin. The mexameter measures the level of melanin, or pigmentation, and erythema, or redness, by reflection. Measurements are arbitrarily expressed as indices of pigmentation. Skin elasticity was performed with the cutometer, based on the well-established suction method. The resistance of the skin to be sucked up by negative pressure (firmness) and its ability to return to its original position (elasticity) are displayed as curves. To analyze skin elasticity, R2 value (gross elasticity: resistance vs. ability of returning) was recorded; R2 increases with increasing skin elasticity. The participants compliance (dosage and way of intake) and tolerance toward the products were checked weekly.

 

Hair Parameter Testing:

Daily scalp hair counts can be useful to the physician to help quantify how much the patient is losing and make sure that this is not more than the physiologic hair loss. It is said that it is normal to lose up to 100 hairs per day (Shapiro J.et al.,2002),. Patients are instructed to collect hairs shed in one day, count them and place them in plastic bags for every week of interval. All shed hairs in the shower or sink or on the brush are collected .If the patient is losing approximately more than 100 hairs per day, then the hair should be examined microscopically to detect any abnormalities.

 

Nail Parameter Testing:

The properties of nails like nail growth, brittleness, flexibility and cracked nails were measured. After the oral consumption of Microcore’s Muttaijow ComplexTM over 5 week period, the growth of nail and its properties were studied after every week interval and the brittleness of nails were also tested (Zague V, et al.,2011). The subjects received a form in which they should record daily how many times their nails had been cracked or chipped on each hand.

 

3. RESULT AND DISCUSSION:

3.1 Compositional analysis of 500 mg of Microcore’s Muttaijow ComplexTM:

Typical analysis of Muttaijow ComplexTM resulted Solubility (20 %), Fat (0 %), Carbohydrate (0 %) and Ash (0 %) was shown in the Table 1. Amino acid composition of Muttaijow ComplexTM for Lysine (2.98%), Tryptophan (2.71%), Leucine (3.96%), Aspartic acid (7.05%), Proline (8.25%), Isoleucine (2.12%), Threonine (4.67%), Glycine (4.01%), Histidine (2.91%), Arginine (5.36%), Tyrosine (1.34%), Glutamic acid (8.25%), Cystine (6.14%), Alanine (2.05%), Methionine (2.85%), Valine (5.15%), Phenylalanine (1.49%), Serine (4.3) were shown in the Table 2. Constituents of Muttaijow ComplexTM resulted 96% protein, 17% Collagen, 27% Elastin (16.2% Desmosine, and 10.8% Iso desmosine), 28.3% Chondroitin sulphate, 20.5% Glycosaminoglycan and 5% Hyaluronic acid was shown in the Table 3.

 

Table 1: Typical analysis of Muttaijow ComplexTM

S. No.

Sample

% Muttaijow ComplexTM

1

Solubility

40

2

Fat

0

3

Carbohydrate

0

4

Ash

0

 

Table 2: Typical Amino acid composition of Muttaijow ComplexTM

S. No.

Aminoacid

% Muttaijow ComplexTM

1

Lysine

2.98

2

Tryptophan

2.71

3

Leucine

3.96

4

Aspartic acid

7.05

5

Proline

8.25

6

Isoleucine

2.12

7

Threonine

4.67

8

Glycine

4.01

9

Histidine

2.91

10

Arginine

5.36

11

Tyrosine

1.34

12

Glutamic acid

8.25

13

Cystine

6.14

14

Alanine

2.05

15

Methionine

2.85

16

Valine

5.15

17

Phenylalanine

1.49

18

Serine

4.3

 

Table 3: Typical constituents of Muttaijow ComplexTM

S. No.

Sample

% Muttaijow ComplexTM

1

Protein

96

2

Collagen (Collagen Type (1,3,5)

17

3

Elastin

27

4

Desmosine

16.2

5

Isodesmosine

10.8

6

Chondroitin sulfate

28.83

7

Glycosaminoglycan

20.15

8

Hyaluronic acid

5

 

3.2 Pilot study-I:

Self-assessment questionnaire responses were analyzed, and results are shown Table 4 and Graph 1,2,3 below. The majority of the participants observed improvement on general face and body skin condition. Of these, 39% and 50% noticed great improvement in general face and body skin condition, respectively. Volunteers’ self-assessment of specific skin properties showed variable percentages, with 33% to 100% of participants observing an improvement. The parameters that received the most positive evaluation (over 50% of participants manifested some positive change) were, in this order; facial skin softness (100%), facial skin hydration (94%), body skin hydration (89%), facial skin brightness (83%), and smoothness of facial skin (72%). Of these, facial skin softness (85%) and body skin hydration (80%) showed great improvement. The effects on hair and nail general condition were valued positively by 67% and 50% of volunteers showed great improvement, respectively. The most outstanding qualities according to volunteers’ self-assessment were hair brightness (56%) and hydration (56%) and nail hardness (61%) showed mild improvement.

 

 

Table 4: Eeffects of Microcore’s Muttaijow ComplexTM according to participants’ self- assessment questionnaires.

S. No.

Parameters

% of Volunteers satisfaction

25% adults of 25-30 years

40% of 30-45 years

27% of 45-60 years

33% of over 60 years

1

Skin Property

 

Facial Skin Hydration

95

95

94

92

 

Facial Skin Brightness

83

82

80

80

 

Smoothness of the Facial skin

79

77

75

72

 

Facial skin softness

100

100

99

98

 

General improvement of facial skin

98

98

95

93

 

Body skin hydration

89

88

85

82

 

General improvement of body skin condition

85

84

83

81

2

Hair Property

 

Hair volume

72

72

70

68

 

Hair thickness

70

70

67

65

 

Hair loss

0

0

1

1

 

General improvement of hair condition

70

71

68

65

3

Nail Property

 

Nail hardness

61

61

59

56

 

Nail faster growth

65

65

63

60

 

Decrease of nail irregularity

72

72

69

67

 

General improvement of nail condition

70

70

65

63

[(70-100%) great improvement, (30-60%) mild improvement, (0%-10%) no change]

 

 

 

Graph 1: Effects of Microcore’s Muttaijow ComplexTM according to participants’ self- assessment questionnaires on skin

 

 

Graph 2: Effects of Microcore’s Muttaijow ComplexTM according to participants’ self- assessment questionnaires on hair

 

Graph 3: Effects of Microcore’s Muttaijow ComplexTM according to participants’ self- assessment questionnaires on Nail

 

3.3 Pilot study-II:

3.3.1 Skin biophysical parameters:

The overwhelming success of Microcore’s Muttaijow ComplexTM treatment, among volunteers participating in the study, led us to conduct a second study aimed at measuring skin parameters such as skin hydration, pigmentation, and elasticity. In total, seven participants completed the study. There were no discomfort or adverse reactions reported. These pilot studies set out to explore the effect of Microcore’s Muttaijow ComplexTM, a dietary supplement consisting of egg membranes, on skin biophysical parameters related to cutaneous aging. Increases in skin hydration and skin elasticity with reduced pigmentation on skin were observed after 5 weeks treatment of Microcore’s Muttaijow ComplexTM supplements than control groups (without intake of oral supplement). Our results are in agreement with previous investigations in which oral administration of collagen-derived products has shown improvement in skin elasticity (Proksch et al., 2014). The improvement observed in skin elasticity suggests a long-lasting positive physiological effect, in contract to effects of topical skin-care products, which increase skin elasticity predominantly by enhancing epidermal hydration (Liu et al., 2015). Moreover, treatment with eggshells has previously shown a positive effect on skin parameters such as pigmentation and hydration. From the results oral intake of 500 mg/day Microcore’s Muttaijow ComplexTM shows significant improvement from initial to after 5 weeks of treatment and also a significant decrease in pigmentation of skin was shown in the Table 5 and Graph 4.

 

3.3.2 Hair biophysical parameters:

500 mg of oral supplement of Microcore’s Muttaijow ComplexTM treated with split ends, hair loss, hair breakage, strong hair scalp improvement. Before and After 5 weeks of treatment (Shapiro J.et al., 2002), there is an significant impact on participants with overwhelming feedback. Improvement in thickened hair growth with reduced split ends, reduction in hair loss and increased in hair strength was shown in the Table 6 and Graph 5. The split ends of hair was measured under the microscope before and after oral supplementation of Microcore’s Muttaijow ComplexTM was shown in the Figure 1.

 

 

Table 5: Effect of Microcore’s Muttaijow ComplexTM in skin parameters

S. No.

Skin Parameters

Time (Weeks)

Control

Initial

Week 1

Week 2

Week 3

Week 4

Week 5

1

Skin hydration

51.4

51.4

52.7

53.5

55.7

59.9

62.4

2

Skin pigmentation

25.6

25.6

23.8

20.6

18.5

16.7

15.1

3

Skin elasticity

71.4

71.4

66.7

68.9

69.5

72.5

79.5

 

Graph 4: Effect of Microcore’s Muttaijow ComplexTM on skin biophysical parameters

 

Graph 5: Effect of Microcore’s Muttaijow ComplexTM on Hair biophysical parameters

 

 

Table 6: Effect of Microcore’s Muttaijow ComplexTM in hair parameters

S. No.

Hair Parameters

Time (Weeks)

Control

Initial

Week 1

Week 2

Week 3

Week 4

Week 5

1

Hair loss

94.5

94.5

94.2

92.8

89.34

85.6

81.3

2

Hair split ends

24.6

24.6

23.8

20.6

19.2

18.9

16.2

 

 

 

Table 7: Effect of Microcore’s Muttaijow ComplexTM in nail parameters

S. No.

Nail Parameters

Time (Weeks)

Control

Initial

Week 1

Week 2

Week 3

Week 4

Week 5

1

Nail growth(cm)

1.1

1.1

1.2

1.4

1.5

1.59

1.7

 

 

Figure 1: Microscopic view of hair at 4X of before and after Treatment with Microcore’s Muttaijow ComplexTM

 

3.3.3 Nail biophysical parameters:

500 mg of oral supplement of Microcore’s Muttaijow ComplexTM treated with participants. Before and after 5 week of treatment, increase of 12% nail growth rate and a decrease of 42% brittle nails within 4 weeks were observed in the Table 7 and Graph 6.

 

Graph 6: Effect of Microcore’s Muttaijow ComplexTM on Nail biophysical parameters

 

Before starting the treatment, the participants’ frequency of broken nails were on average 10 times/mo It significantly decreased to 6 times/mo after 5 weeks of treatment, representing a reduction of 60%. The ingestion of Microcore’s Muttaijow ComplexTM led to a global clinical improvement in brittle nails, a significant increase in nail growth rate, and a decrease in the frequency of cracked or chipped nails in the present trial after 5 weeks of treatment.

 

4. CONCLUSIONS:

A pilot studies on oral supplementation of 500 mg/day of Microcore’s Muttaijow ComplexTM for 12 weeks has been conducted to investigate the effect of on skin, hair and nail health by self-assessment questionnaires and their properties are studied. The following results were observed from the Pilot studies. In the study 1, self-assessment questionnaire are investigated to check the effect of Muttaijow ComplexTM on skin, hair, and nails. It was observed that participants were predominantly satisfied with the beneficial effects on skin health. Volunteers shown 95% satisfaction on skin hydration and skin softness on face, perhaps 83% of volunteers were satisfied with facial skin brightness and overall body skin condition. Around 79% of volunteers shown a satisfactory result on body skin smoothness. Almost 70% of people were shown a good development in hair volume, thickness and overall hair richness. There was also a significant improvement in nail and the volunteers of 65-70% gave development of clean nail and reduced white and gray lines. From the pilot study 2 it was noted that the significant improvement in skin elasticity was absorbed after the 5-week with Microcore’s Muttaijow ComplexTM treatment. Collagen type-1 is being a key molecule of skin as it is found in Microcore’s Muttaijow ComplexTM and that plays an important role in maintaining its structure and hydration. Thus the study concludes that oral intake of Microcore’s Muttaijow ComplexTM led to a significant increase in skin hydration and skin elasticity with reduced pigmentation on skin were observed after 5 weeks treatment, which is a very important marker for skin aging. Microcore’s Muttaijow ComplexTM also shows significant impact on hair thickness, reduces split ends, hair loss, hair breakage and improvement in hair growth. Above 75% of hair loss has been reduced after 5 week of oral intake of Muttaijow ComplexTM. Improvement in nail physiology with increase of 12% nail growth rate and a decrease of 42% brittle nails within 5 weeks. Thus Microcore’s Muttaijow ComplexTM achieving a significant changes in skin, hair, nails than control group (without oral supplements intake). From the result analysis, Microcore’s Muttaijow ComplexTM is deemed to be one of the most effective anti-aging products in cosmetic sector. Muttaijow ComplexTM has been shown to naturally contain a number of essential components such as collagen, HA, and glucosamine that are found in the skin and have proven a good dermatologic effects. Oral supplementation of 500mg/day of Microcore’s Muttaijow ComplexTM 12 weeks is associated with significant improvement in the appearance of facial skin, hair, nail growth and improvement of brittle nails in conjunction with a notable decrease in the frequency of broken nails.

 

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Received on 24.09.2020            Modified on 21.10.2020           

Accepted on 29.11.2020              ©A&V Publications all right reserved

Research J. Topical and Cosmetic Sci. 2021; 12(1):31-38.

DOI: 10.52711/2321-5844.2021.00005