INTRODUCTION:

Hair is an epidermal derivative which is one of the vital parts increasing the overall elegance of the body. Hair fall, dandruff, lice, spilt ends, grey hair are few problems involved with hair faced by human.¹ To overcome these, human takes many measures by applying many cosmetics for each. Hair oil is one among them used to solve almost all of this problems.² Hair care cosmetics are now added with herbs and they are well recognised compared with synthetic ones.³ Herbal hair oil is more preferred and is used in many ailments of hair.⁴They promote hair growth, improve elegance of hair and prevent hair fall.⁵ Hair oil not only promotes hair growth they also provide necessary moisture to the scalp rendering in beautiful hair.⁶ Herbal cosmetics have growing demand in the world market and are in an invaluable gift of nature. There are a wide range of herbal cosmetics products to satisfy beauty regime. Adding herb in the cosmetics is very safe for our skin.^{7, 8, 9}

Collection of Plant Materials:

The polyherbal hair oil was prepared by collecting various plant materials like, Onion , Lemon Peels, Lemon Grass, Mustard, Neem, Nirgundi, Hibiscus, Garlic, Jasminum officinale, Olive Oil, Camphor, Grape seed oil were procured from local market.

Onion

Synonyms : Onion plant, Allium cepa.

Biological Source: Allium cepa

Family : Alliaceae

Use: It reduce hair loss by improving blood circulation in the scalp and hair. the sulphur in the juice helps in the production of essential collagen that promotes hair growth.

Fig. no.1-Onion

Lemon Peels:

Synonyms : Citrus. Citrus fruit.

Biological source : Citrus limon

Family : Rutaceae

Uses:

Promote hair growth, it cleanses and balances the pH of the scalp, the antimicrobial properties will assist with any latent fungus, while acne and dandruff will find relief from its antiseptic benefits.

Fig. no. 2–Lemon peels

Lemon Grass:

Synonyms:

Indian oil of Verbena, Indian Melissa oil, Mar-Hirvacha tel

Biological source:

Lemon grass oil is the oil distilled from Cymbopogan flexuosus Stapf or Cymbopogan citrates Staf.

Family : Graminae

Uses:

Lemongrass oil is one of the beneficial essential oils that helps regrow hair by addressing any scalp issue that are affecting healthy hair growth such as eczema, dandruff, and psoriasis.

Fig. no.3- Lemon Grass

Mustard:

Synonym: Brown Mustard.

Biological Sources:

These are dried ripe seeds of Brassica nigra Linn., Koch or Brassica juncea Linn.

Family : Cruciferae

Uses:

Nourishes for Hair, Natural Conditioner, Promotes Hair Growth, Anti-Fungal Properties.

Fig. no.4-Mustard

Neem:

Synonyms: Melia Azadirachta. Margosa.Azadirachta indica.

Biological Sources: Neem seed. Neem tree.

Family: Meliaceae

Botanical name: Azadirachta Indica.

Use: It reduce hair loss by improving blood circulation in the scalp and hair. the sulphur in the juice helps in the production of essential collagen that promotes hair growth.

Fig. no.5- Neem

Nirgundi

Synonym: Vitex cannabifolia Siebold and Zucc Vitex incisa Lam, Vitex incisa var. heterophyllaFranch.

Botanical Name: Vitex Negundo

Family Name: Verbenaceae

Uses: Aids in Hair Growth: The oil made from the leaves of this tree can be used as a hair tonic.

Regular application is known to rejuvenate the hair, prevent hair fall, and promote hair growth.

Fig. no.6-Nirgundi

Hibiscus:

Synonyms: Bombycidendron Zoll. and Moritzi, Bombycodendron Hassk, Brock mania W. Fitzg.

Family: Malvaceae

Uses: Boosts Up Hair Growth, Thwarts Premature Graying and Ageing, Excellent for Treating Dandruff, Makes Hair Thicker and Stronger. Nourishes the Hair by Conditioning It.

Fig. no.7-Hibiscus

Garlic:

Synonyms: Allium sativum L

Biological Source: Bulbs of Allium sativum

Family: Lilaceae

Use: Garlic could end your hair loss problems because of its high levels of allicin,a sulphur found to effectively treat hair loss.

Fig. no.8-Garlic

Formulation of Oil Formulation table

Table no.1-Ingredients used in formulation of herbal hair oil (formulated)

S. No.	Ingredients	Quantity (%)
1	Onion oil	5ml
2	Lemon Bulb oil	5ml
3	Lemon grass oil	5ml
4	Mustard oil	5ml
5	Neem oil	5ml
6	Nirgundi oil	5ml
7	Hibiscus oil	5ml
8	Garlic oil	5ml
9	Jasmine oil	5ml
10	Olive oil	5ml
11	Camphor oil	5ml
12	Grape seed oil	5ml

First collected all oils or ingredients. Then measured 5 ml of all oils or ingredients are taken with the help of measuring cylinder. Then all oils or ingredients are mixed in a beaker. Then prepared mixture is filter. This mixture is poured in suitable container. Then Evaluate and submit our prepared hair oil. Then the prepared hair oil is compared with marketed formulation.

Evaluation of Herbal Hair Oil¹⁰

Sensitivity test:

The prepared herbal hair oil was applied on 1 cm skin of hand and exposed to Sunlight for 4-5 min.

Acid value:

Preparation of 0.1 molar solution: Weighed 0.56 g KOH pellets and dissolved in 100 mL of distilled water and stirred continuously. The prepared 0.1 molar KOH solution was filled in the burette. Preparation of sample: Measured 10 mL oil and dissolved in 25 mL of ethanol and 25 mL of ether mixture and shacked. Added 1 mL of phenolphthalein solution and titrated with 0.1 molar KOH solution.

Saponification value:

Accurately weighed 1 mL of oil into a 250 mL of conical flask and 10 mL of ethanol: ether mixture (2: 1) was added. To this flask 25 mL of 0.5 N alcoholic KOH was Kept the flask for 30 min. and the flask was cooled. The cooled solution was titrated against 0.5 N HCl using phenolphthalein indicator. Similarity the blank titration was performed without taking oil (sample). Amount of KOH in mg used was calculated.

_PH:

The pH of herbal hair oil was determined using pH meter.

Viscosity:

The viscosity was determined using Ostwald’s viscometer.

Specific gravity:

Take the specific gravity bottle, rinsed it with distilled water, dry it in oven for 15 minutes, cool, closed it with cap and weigh it (a). Now fill the same specific gravity bottle with the sample and closed it with cap and again weigh it (b). Determine the weight of sample per milliliter by subtracting the weight (b-a).

Table no.2- Evaluation of herbal hair oil (Prepared)

S. No.	Parameter	Inference
1.	Specific gravity	1.082
2.	Viscosity	0.94
3.	Acid value	4.4
4.	Saponification value	111.03
5.	pH	6.7
6.	Sensitivity test	No irritation
7.	Irritation test	No irritation
8.	Grittiness	Smooth

Table no.3-Evaluation of herbal hair oil (marketed)

S. No.	Parameter	Inference
1.	Specific gravity	1.096
2.	Viscosity	0.93
3.	Acid value	4.5
4.	Saponification value	115.05
5.	pH	7.2
6.	Sensitivity test	No irritation
7.	Irritation test	No irritation
8.	Grittiness	Smooth

Table no.4-Role of herbs in herbal hair oil (Prepared)

S. No.	Ingredients	Importance
1	Onion	Hair growth
2	Lemon	Anti-Dandruff
3	Lemon grass	Cure Hair loss
4	Mustard oil	Blood circulation, scalp Hair growth
5	Neem	Hair growth, Anti-Dandruff
6	Nirgundi	Hair Growth
7	Hibiscus	Hair growth
8	Garlic	Hair Growth
9	Jasmine	Antiseptic
10	Olive oil	Hair Growth, Anti-Dandruff, Anti-fungus
11	Camphor	Hair growth
12	Grape seed oil	Vit E, Hair Growth

Table no.5-Role of herbs in herbal hair oil (marketed)

S. No.	Ingredients	Importance
1	Argan oil	Increase hair's elasticity and consistently restore shine to dull
2	Sesame oil	Hair growth
3	Almond oil	Cure Hair loss
4	Olive oil	Blood circulation n scalp, Hair growth
5	Sea buckthorn oil	Hair growth, Anti-Dandruff
6	Murumuru oil	Hair Growth
7	Jojoba oil	Cure Hair loss
8	Indian Goose Berry oil	Hair Growth
9	Fenugreek oil	Antiseptic
10	Rosemary oil	Hair Growth, Anti-Dandruff, Anti-fungus
11	Spearmint oil	Hair growth
12	Vitamin E oil	Hair growth

RESULTS AND CONCLUSION:

Herbal hair oil is one of the most well recognized hair treatments. Herbal hair oil not only moisturizes scalp but also reverses dry scalp and dry hair condition. It provides numerous essential nutrients required to maintain normal function of sebaceous glands and promotes natural hair growth. The herbal hair oil was prepared from various herbs (Table 1) and their importance in the formulation is presented in (Table 4). The various parameters like sensitivity test, viscosity, pH, irritation test, grittiness test, saponification value and acid value of herbal hair oil was evaluated (Table 2 and 3). Hence, from the present investigation it was found that the formulated herbal hair oil has optimum standards and further standardization and biological screening establishes the efficacy of formulated herbal hair oil

Table no.6-Result of formulation

Sr. No.	Evaulation Test	Prepared Hair Oil	Marketed Hair Oil
1	Sensitivity test	No irritation	No irritation
2	Acid value	4.4	4.5
3	Saponification value	111.03	115.05
4	pH	6.7	7.2
5	Viscosity	0.94	0.93
6	Specific gravity	1.082	1.096

CONCLUSION:

From the obtained result we were conclude that each and every prepared hair oil and marketed hair oil are similar to each other. Evaluation gives different results.

ACKNOWLEDGEMENT:

Authors are highly Acknowledge the help of teaching staff of Rajarambapu College of Pharmacy, Kasegaon. For providing necessary information required for research work. Also we are highly acknowledge the help and guidance of Dr. Mangesh A. Bhutkar.

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Received on 21.05.2019 Accepted on 28.05.2019

Research J. Topical and Cosmetic Sci.10(1):09-12.

DOI: 10.5958/2321-5844.2019.00003.7