Formulation, development and comparative study of facial scrub using synethetic and natural exfoliant.

 

Pranjali Talpekar*, Monica Borikar

Department of Cosmetic Technology, Somalwars Nikalas Womens College, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur, India

*Corresponding Author E-mail: amitbhople2@gmail.com

 

ABSTRACT:

For healthy skin; regular cleansing of skin to remove dirt, dead cell, sebum etc was necessary. Facial Scrub contain Natural/Synthetic exfoliant give exfoliation effect remove dead cell etc. In this study; formulation and development of Face Scrubs using Natural exfoliant (Jojoba meal) and Synthetic exfoliant (Polyethylene Beads) in concentration 1%, 1.5% and 2%. Comparative study between Scrubs with Natural and Synthetic exfoliant was did for exfoliation effect. Scrub evaluated for Stability Study at Room temp (27C), Cold temp (4C) and accelerated temp (45C), Spreadability and Total Fatty Matter (TFM) content. Subject test on 20 healthy volunteers was done to examine Skin irritation and dead cell removal property of scrub. Dead cell removal property studied by Tape Stripping method. Both Face Scrubs found stable in stability study. Scrubs also passed the Spreadability and Total Fatty Matter (TFM) content test. On basis of subject study and tape stripping method; Face scrub with Synthetic exfoliant i.e. Polyethylene Beads in 2% concentration shows better exfoliation effect which is useful for oily skin. Face Scrub with Natural Exfoliant i.e. Jojoba meal in 2% concentration found mild and gentle exfoliation effect which beneficial foe sensitive and dry skin.

 

KEYWORDS: Exfoliation, Natural exfoliant, Synthetic exfoliant, Subject Study, Facial scrub.

 

 


INTRODUCTION:

One of largest organ of Body i.e. Skin function as a protective wrapper, keeping everything underneath it safe from daily threats such as the harsh effects of sun, wind, pollution and germ-filled grime1.

 

For healthy Skin and its good appearance, regular cleansing of Skin surface require to remove dirt, grime, dead cells, crusts, make ups, sebum and other secretions1.

 

In Exfoliation process, removal of the oldest superficial dead cells from skin's outermost surface is done. Exfoliation can achieve by Chemical and Mechanical means. Exfoliation by mechanical means is accomplished by physically scrubbing skin with abrasive. Routine exfoliation to skin lead to natural shedding process of skin results in smoother, tones and healthier skin. Skin exfoliation give benefits in Acne vulgaris, clogged pores and Wrinkles1.

 

Skin care scrub products, also called skin or body polishes are a unique category of cleansing material called exfoliating cleansers. They focus on cleansing, conditioning and treating1.

 

 

Facial scrubs are cleansers. They generally contain small particles of natural or polymeric ingredients. Scrubs are intended to provide a deep cleansing experience including a higher level of skin exfoliation from abrasion with the particles. A non - exhaustive list of natural scrub particles includes seeds of many fruits (e.g. peach, apple, apricot), nut shells (e.g. almond, walnut), grains (e.g. oats, wheat), and sandalwood. Synthetic scrub particles include polyethylene or polypropylene beads2.

 

In addition to the exfoliant, a face scrub usually contains a carrier oil or cream which is used to hold the exfoliant in a suspension, along with a scent of some kind, often provided by an essential oil which is also supposed to promote relaxation or well-being2.

 

Facial scrubs are made with a mild abrasive which is designed to pull away dead and dying skin3.

 

Facial scrubs contain wide range of exfoliants range from Natural to Synthetic. There are wide ranges of exfoliants available in the market such as polyethylene beads and jojoba meal.

 

In this investigation, Comparative study of Facial Scrub by using Synthetic and Natural exfoliants was studied. Facial different Scrubs, one formulated with Synthetic exfoliant and other formulated with Natural exfoliant were evaluated for better exfoliation effect.

 

MATERIAL AND METHOD:

Polyethylene Beads obtained from Anshul Life Sciences Pvt. Ltd.; Mumbai; India., Jojoba meal obtained from Yash Export Ltd., Jaipur; India. Other ingredients purchase from License Manufacturer in India.

 

Formulation and Development of Face Scrub

Formulation of Scrub Base

Three batches i.e. B1, B2 and B3 of Scrub base were formulated by varying the quantity of ingredients as shown in Table I.

 


 

 

Table I. Base of Face Scrub

Ingredients

Quantity 100%

B1 B2 B3

Phase A Stearic acid 7% 8% 8.5%

 

Mineral Oil 8 8 8

Lanolin 1 1 1

Glyceryl 3 3 3

monostearate

Cetyl alcohol - 1.5 2

Propyl paraben 0.2 0.2 0.2

Isopropyl myristate 2 2 2

Almond oil 1 1 1

Phase B Propylene glycol 4 4 4

 

Triethanolamine 1 1.5 1.5

Water q.s 64.8 63.5

Glycerin 4 4 4

Methyl paraben 0.2 0.2 0.2

Phase C Sodium lauryl ether sulphate 0.5 0.7 1

Phase D Lemon oil 0.1ml 0.1 ml 0.1ml

 

 

 

 


Procedure for Preparation of Face Scrub Base

Weigh all ingredients of Phase A and Phase B accurately. Heat both Phases A and B separately on water bath up to 75-80 C. Add Phase A to Phase B with continuous stirring; transfer it to mortar triturate thoroughly to form emulsion. Add Phase C in mortar with trituration. Lastly add Phase D 45 C and triturate thoroughly to form uniform emulsion.

 

 

Formulation and Preparation of Face Scrub

In formulation of Face Scrub, addition of Active (Exfoliant) was added to Face Scrub Base Batches B1, B2 and B3. In this study, two different Exfoliants were used i.e. Polyethylene Beads an Synthetic exfoliant and Jojoba meal a Natural exfoliant.

 

 

 

Preparation of Face Scrub with Synthetic Exfoliant i.e. Polyethylene Beads

Addition of Polyethylene Beads into the base, mix them thoroughly in Homogenizer for uniform mixing. Formulation of Three batches of Face Scrub varies in concentration of Polyethylene Beads i.e. 1%, 1.5% and 2% were prepared (Table II).

 


 

 

Table II. Formulation of Face Scrub with Synthetic Exfoliant i.e. Polyethylene Beads

 

Ingredients

Quantity 100%

PB1 PB2 PB3

Phase A Stearic acid 7 8 8.5

 

Mineral Oil 8 8 8

Lanolin 1 1 1

Glyceryl monostearate 3 3 3

Cetyl alcohol -- 1.5 2

Propyl paraben 0.2 0.2 0.2

Isopropyl myristate 2 2 2

Almond oil 1 1 1

Phase B

Propylene glycol 4 4 4

Triethanolamine 1 1.5 1.5

Water q.s. 64.8 63.5

Glycerin 4 4 4

Methyl paraben 0.2 0.2 0.2

Phase C Sodium lauryl ether sulphate 0.5 0.7 1

Phase D Lemon oil 0.1 0.1 0.1

 

Polyethylene beads 1 1.5 2

Perfume 0.1 ml 0.1 ml 0.1 ml

 

 

 


Preparation of Face Scrub with Natural Exfoliant i.e. Jojoba Meal

Addition of Jojoba meal into the base, mix them thoroughly in Homogenizer for uniform mixing. Formulation of Three batches of Face Scrub varies in concentration of Jojoba Meal i.e. 1%, 1.5% and 2% were prepared (Table III).

 


 

 

Table III. Formulation of Face Scrub with Synthetic Exfoliant i.e. Jojoba meal

Ingredients

Quantity 100%

JB1 JB2 JB3

Phase A Stearic acid 7 8 8.5

 

Mineral Oil 8 8 8

Lanolin 1 1 1

Glyceryl monostearate 3 3 3

Cetyl alcohol -- 1.5 2

Propyl paraben 0.2 0.2 0.2

Isopropyl myristate 2 2 2

Almond oil 1 1 1

Phase B Propylene glycol 4 4 4

 

Triethanolamine 1 1.5 1.5

Water q.s. 64.8 63.5

Glycerin 4 4 4

Methyl paraben 0.2 0.2 0.2

Phase C Sodium lauryl ether 0.5 0.7 1

sulphate

Phase D Lemon oil 0.1 0.1 0.1

 

Jojoba meal 1 1.5 2

Perfume 0.1 ml 0.1 ml 0.1 ml

 

 


 

Evaluation of finished product (facial scrub)

Determination of spreadability of scrub

To determine the spreadability caused by abrasive scrub (0.1gm), it was placed on a slide and another slide slided over it, which had 200gm weight placed over it. Spreadability was calculated4.

 

Determination of Total Fatty Substance content

Weigh accurately about 2gm of the material into a conical flask, add 25ml of dilute Hydrochloric acid, fit a reflux condenser into a flask, and boil the content until the solution is perfectly clear. Pour the content of the flask into a 300ml separating funnel and allow it to cool to 20C. Rinse the conical flask with 50ml of ethyl ether rinsing into the separating funnel; shake well and leave until the layers separate.

 

Separate out the aqueous phase and shake it out with 50ml portions of ether twice. Combine all the ether extracts and wash with water until free of acid (when tested with methyl orange indicator solution). Filter the ether extracts through filter paper containing sodium sulphate into a conical flask. Wash sodium sulphate on filter paper with ether and combine the washing with filtrate. Distill off the ether and dry the material remaining in the flask at temperature of 60 2C to constant mass5.

 

Total Fatty Substance, % by mass= 100 (M1/M2)

Where;

M1 mass in grams of residue

M2 mass in grams of material taken for the test.

 

Stability Study of Finished Product

Stability Study of Finished Product was carry out at various temperature i.e. Room temperature 27C; Cold temperature 4C and accelerated temperature 45C. During stability study, stability parameter colour, odour and pH of Finished product is evaluated5.

 

Determination of Colour Change

The colour change was observed visually. The colour of the sample was noted regularly at various temperatures (4C, 45C, and 27C) and at the interval of 2 days and the results was evaluated5.

 

Determination of Odour change

The odour change was noted by smelling the product. The odour of the sample were noted regularly at various temperatures (4C, 45C, and 27C) and at the interval of 2 days and the results was evaluated5.

 

 

 

Determination of pH

Weigh 5 gm of the scrub in beaker. Add 45 ml water in it and disperse properly. Determine the pH at 27C using pH meter5.

 

Subject Study

There are 20 Healthy Volunteers age between 22-31 years were involved. All Volunteers were selected to subjective study to determine effectiveness of formulation with regards to their ability to remove dead cells.

 

Subject Study Protocol

All 20 Volunteers participate in study were not suffering from any skin disease and skin disorder. All subjects were informed not to use cleansing or skin care product for one week prior and during period of study. Two Optimize samples of formulation i.e. Sample A (Face Scrub with Polyethylene beads) and Sample B (Face Scrub with Jojoba meal) were applied topically by 20 subjects for two weeks; each sample applied once in week. Application of Sample A followed by Application of Sample B on alternate day. Parameters like Skin irritation and Removal of dead cell are evaluated. Skin irritation evaluated from subjects response to sample. Removal of dead cell parameter was evaluated by Tape Stripping Method.

Tape Stripping Test

Tape stripping is a simple and efficient method for the assessment of quality and efficacy of cosmoceuticals and dermatological formulations. In this method, Stripping tape applied over predetermines area which removes cell lines of stratum corneum along with it after pull off the tape. The separated Stratum corneum cell line adhering to tape evaluated microscopically6.

 

In this investigation; exfoliation effect of optimized Scrub i.e. Sample A (Face Scrub with Polyethylene beads) and Sample B (Face Scrub with Jojoba meal) by tape stripping method was evaluated. Three independent sites S1, S2 and S3 separated by 5 cm over face were selected. Site S1 is blank; no any sample of Scrub applied over it. Sample A (Face Scrub with Polyethylene beads) and Sample B (Face Scrub with Jojoba meal) apply over Site S2 and S3 respectively.

 

Apply the adhesive tape over Site S1; pull off the tape. Add drops of methylene blue on it; dry it. After drying pour water over it until methylene blue is washed off. Dry the tape and observe under microscope to evaluate cell line adhering to tape.

 

 

Same procedure was followed for Face scrub sample A and B. Sample A (Face Scrub with Polyethylene beads) and Sample B (Face Scrub with Jojoba meal) apply over Site S2 and S3 respectively. Apply the adhesive tape over Site S2 and S3; pull off the tape. Add drops of methylene blue on it; dry it. After drying pour water over it until methylene blue is washed off. Dry the tape and observe under microscope to evaluate cell line adhering to tape7.

 

RESULT AND DISCUSSION:

Determination of spreadability of Face scrub

The spreadability of Face Scrub with Polyethylene beads; Batch PB3 has maximum spreadability among three batches (Table IV). In Face Scrub with Jojoba meal; Batch JB3 has maximum spreadability among three batches (Table IV).

Table IV. Spreadability of Face scrub

Sr. No. Batch Spreadability (Area Calculated as cm2)

L B A

Spreadability of Scrub with Polyethylene beads

01 PB1 2.2 cm 1.0 cm 2 cm2

02 PB2 2.2 cm 1.1 cm 2.42 cm2

03 PB3 2.5 cm 1.2 cm 3 cm2

Spreadability of Scrub with Jojoba meal

01 JB1 2.7 cm 1.6 cm 4.32 cm2

02 JB2 2.9 cm 1.8 cm 5.22 cm2

03 JB3 3.1 cm 2.1 cm 6.51 cm2

L: Length, B: Breadth; A: Area

 

 

Determination of Total Fatty Matter (TFM) content

Total Fatty Material (TFM) Value all the batches of Face Scrub (Table V) were all above the minimum required limit.

 

 

 

Table V. Total Fatty Matter (TFM) of Face Scrub

Sr. No. Batch Total Fatty Matter (TFM)

TFM of Scrub with Polyethylene beads

01 PB1 22.41%

02 PB2 22.64%

03 PB3 22.88%

TFM of Scrub with Jojoba meal

01 JB1 25.05%

02 JB2 25.29%

03 JB3 25.46%

 

 

 

Stability Study of Finished Product

Colour Change

No colour change was observed in Facial Scrub with Polyethylene beads at 4C, 45C and 27C, scrub found stable (Table VI). Slightly Colour change occurs in Facial Scrub with Jojoba meal Batch JB2 at 4C which is negligible, scrub found stable (Table VI).

Table VI. Parameter Colour Change of Facial Scrub in Stability Study

Days Room Temp. Cold Temp. Accelerated Temp.

(27C) (4C) (45C)

Face Scrub with Polyethylene beads

Batch No. PB1 PB2 PB3 PB1 PB2 PB3 PB1 PB2 PB3

1 OC OC OC OC OC OC OC OC OC

3 OC OC OC OC OC OC OC OC OC

5 OC OC OC OC OC OC OC OC OC

7 OC OC OC OC OC OC OC OC OC

9 OC OC OC OC OC OC OC OC OC

11 OC OC OC OC OC OC OC OC OC

13 OC OC OC OC OC OC OC OC OC

15 OC OC OC OC OC OC OC OC OC

17 OC OC OC OC OC OC OC OC OC

19 OC OC OC OC OC OC OC OC OC

21 OC OC OC OC OC OC OC OC OC

23 OC OC OC OC OC OC OC OC OC

25 OC OC OC OC OC OC OC OC OC

27 OC OC OC OC OC OC OC OC OC

29 OC OC OC OC OC OC OC OC OC

30 OC OC OC OC OC OC OC OC OC

Face Scrub with Jojoba meal

Batch No. JB1 JB2 JB3 JB1 JB2 JB3 JB1 JB2 JB3

1 OC OC OC OC OC OC OC OC OC

3 OC OC OC OC OC OC OC OC OC

5 OC OC OC OC OC OC OC OC OC

7 OC OC OC OC OC OC OC OC OC

9 OC OC OC OC OC OC OC OC OC

11 OC OC OC OC OC OC OC OC OC

13 OC OC OC OC OC OC OC OC OC

15 OC OC OC OC OC OC OC OC OC

17 OC OC OC OC OC OC OC OC OC

19 OC OC OC OC OC OC OC OC OC

21 OC OC OC OC OC OC OC OC OC

23 OC OC OC OC OC OC OC OC OC

25 OC OC OC OC SCC OC OC OC OC

27 OC OC OC OC SCC OC OC OC OC

29 OC OC OC OC SCC OC OC OC OC

30 OC OC OC OC SCC OC OC OC OC

C: Original colour, VSCC: Very slight colour change, SCC: Slight colour change, CC: Colour change.

 

Odour Change

No Odour change found in both Facial Scrub i.e. Facial Scrub with Polyethylene beads and Facial Scrub with Jojoba meal at 4C, 45C and 27C, Finished Product found stable (Table VII).

 

 

Table VII. Parameter Odour Change of Facial Scrub in Stability Study

Days Room Temp. Cold Temp. Accelerated Temp.

(27C) (4C) (45C)

Face Scrub with Polyethylene beads

Batch No. PB1 PB2 PB3 PB1 PB2 PB3 PB1 PB2 PB3

1 OO OO OO OO OO OO OO OO OO

3 OO OO OO OO OO OO OO OO OO

5 OO OO OO OO OO OO OO OO OO

7 OO OO OC OO OO OO OO OO OO

9 OO OO OC OO OO OO OO OO OO

11 OO OO OC OO OO OO OO OO OO

13 OO OO OC OO OO OO OO OO OO

15 OO OO OC OO OO OO OO OO OO

17 OO OO OC OO OO OO OO OO OO

19 OO OO OC OO OO OO OO OO OO

21 OO OO OC OO OO OO OO OO OO

23 OO OO OC OO OO OO OO OO OO

25 OO OO OC OO OO OO OO OO OO

27 OO OO OC OO OO OO OO OO OO

29 OO OO OC OO OO OO OO OO OO


30 OO OO OC OO OO OO OO OO OO

 

 

Table VII. Parameter Odour Change of Facial Scrub in Stability Study

Face Scrub with Jojoba meal

Batch No. JB1 JB2 JB3 JB1 JB2 JB3 JB1 JB2 JB3

1 OO OO OO OO OO OO OO OO OO

3 OO OO OO OO OO OO OO OO OO

5 OO OO OO OO OO OO OO OO OO

7 OO OO OO OO OO OO OO OO OO

9 OO OO OO OO OO OO OO OO OO

11 OO OO OO OO OO OO OO OO OO

13 OO OO OO OO OO OO OO OO OO

15 OO OO OO OO OO OO OO OO OO

17 OO OO OO OO OO OO OO OO OO

19 OO OO OO OO OO OO OO OO OO

21 OO OO OO OO OO OO OO OO OO

23 OO OO OO OO OO OO OO OO OO

25 OO OO OO OO OO OO OO OO OO

27 OO OO OO OO OO OO OO OO OO

29 OO OO OO OO OO OO OO OO OO

30 OO OO OO OO OO OO OO OO OO

OO: Original odour, VSOC: Very slight odour change, SOC: Slight odour change; CC: Odour change

 

 

Determination of pH

Very slight change in pH of Facial Scrub (Table VIII) occur which is within limit, Finished product found stable. pH change illustrated in Figure 1 and 2.

 

Table VIII. Parameter pH Change of Facial Scrub in Stability Study

Days Room Temp. Cold Temp. Accelerated Temp.

(27C) (4C) (45C)

Face Scrub with Polyethylene beads

Batch No. PB1 PB2 PB3 PB1 PB2 PB3 PB1 PB2 PB3

1 6.30 6.29 6.30 6.29 6.30 6.30 6.37 6.36 6.30

3 6.30 6.30 6.32 6.30 6.32 6.30 6.35 6.43 6.32

5 6.32 6.32 6.35 6.30 6.30 6.30 6.43 6.30 6.35

7 6.35 6.30 6.35 6.32 6.29 6.30 6.30 6.38 6.35

9 6.33 6.36 6.40 6.31 6.30 6.30 6.38 6.30 6.40

11 6.30 6.31 6.42 6.36 6.30 6.30 6.30 6.34 6.45

13 6.30 6.30 6.42 6.30 6.30 6.30 6.40 6.30 6.46

15 6.38 6.30 6.50 6.30 6.30 6.30 6.35 6.41 6.32

17 6.32 6.30 6.51 6.31 6.33 6.30 6.30 6.40 6.30

19 6.30 6.32 6.52 6.36 6.32 6.30 6.32 6.37 6.37

21 6.31 6.38 6.55 6.31 6.30 6.32 6.37 6.30 6.35

23 6.30 6.30 6.55 6.31 6.30 6.32 6.46 6.35 6.42

25 6.30 6.30 6.55 6.30 6.33 6.32 6.30 6.30 6.30

27 6.37 6.35 6.60 6.30 6.30 6.32 6.42 6.33 6.31

29 6.32 6.30 6.60 6.30 6.30 6.35 6.36 6.30 6.35

30 6.32 6.30 6.60 6.30 6.30 6.35 6.30 6.30 6.30

Face Scrub with Jojoba meal

Batch No. JB1 JB2 JB3 JB1 JB2 JB3 JB1 JB2 JB3

1 5.97 5.96 5.94 5.98 6.08 6.07 6.13 6.15 5.99

3 6.07 5.97 5.94 6.02 6.12 6.21 6.00 6.07 5.97

5 6.03 6.03 5.97 6.04 6.00 6.21 6.00 6.03 6.02

7 6.00 6.07 5.97 5.98 6.04 6.15 6.02 6.12 6.21

9 5.95 5.99 6.00 5.97 5.96 5.94 6.00 6.07 5.97

11 5.99 6.14 6.05 6.03 6.03 5.97 5.94 6.00 6.12

13 6.00 6.08 6.1 6.04 6.00 6.21 6.03 5.97 6.01

15 6.03 6.13 6.1 6.16 6.09 6.14 6.21 6.15 6.08

17 5.98 6.04 6.15 6.11 6.04 5.94 6.16 6.03 6.18

19 6.00 5.96 6.15 5.98 6.08 6.07 6.09 6.17 6.05

21 6.09 6.07 6.19 6.07 6.17 6.20 6.11 5.17 5.94

23 6.04 6.00 6.21 6.13 6.15 5.99 6.04 6.00 6.21

25 5.94 6.04 6.23 6.00 6.03 6.02 5.95 5.99 6.00

27 6.02 6.12 6.21 5.95 5.99 6.00 5.98 6.08 6.07

29 6.07 6.05 6.18 6.03 6.03 5.97 6.09 6.07 6.19

30 5.98 6.08 6.07 6.00 6.07 5.97 6.00 6.08 6.1

 

 

 

 

Figure 1. pH change in Stability Study for Face Scrub with Polyethylene beads

 

 

 

Figure 2. pH change in Stability Study for Face Scrub with Jojoba meal

 

 

Selection of Optimized formulation

The Sprediability of Face Scrub Batch PB3 and JB3 was maximum. TFM of Batch PB3 and JB3 was well above the minimum required limit. In stability study; Batch PB3 and JB3 were found stable. Hence in Scrub with Polyethylene beads, Batch PB3 and Scrub with Jojoba meal; Batch JB3 was selected as optimized formulation. Scrub with Polyethylene beads, batch PB3 as Sample A and Scrub with Jojoba meal; Batch JB3 as Sample B use in subject study.

 

 

Subject Study

In Subject Study; only two subject with Sample A (Scrub with Polyethylene beads) and three subject with Sample B (Scrub with Jojoba Meal) were report irritation (Table IX). Number of subject reported irritation was negligible; product consider being safe. Removal of dead cell analyzed by Tape Stripping method. Two subject with Sample A shows negative result for Removal of Dead Cells (Table IX). Four subjects with Sample B show negative result for Removal of Dead Cells (Table IX). Hence Sample A i.e. Scrub with Polyethylene beads shows better Dead cell removal property compare to Sample B (Scrub with Jojoba Meal). Scrub with Polyethylene beads shows better exfoliation compare to Scrub with Jojoba Meal which have mild exfoliation effect.

 

 

Table IX. Subject Test Results

Sr. No. Subject Age Irritation Removal of Dead Cells

1W 2W 1W 2W

Sample A: Face Scrub with Polyethylene beads

1.       S1 23 N N Y Y

2.       S2 24 N N N N

3.       S3 26 N N Y Y

4.       S4 30 Y Y Y Y

5.       S5 30 N N Y Y

6.       S6 27 N N Y Y

7.       S7 27 N N Y Y

8.       S8 24 Y Y Y Y

9.       S9 26 N N Y Y

10.     S10 23 N N Y Y

11.     S11 28 N N Y Y

12.     S12 30 N N Y Y

13.     S13 31 N N N N

14.     S14 24 N N Y Y

15.     S15 24 N N Y Y

16.     S16 25 N N Y Y

17.     S17 25 N N Y Y

18.     S18 24 N N Y Y

19.     S19 25 N N Y Y

20.     S20 25 N N Y Y

Sample B: Face Scrub with Jojoba meal

1.       S1 23 N N Y Y

2.       S2 24 Y Y Y Y

3.       S3 26 N N Y Y

4.       S4 30 N N Y Y

5.       S5 30 N N Y Y

6.       S6 27 N N Y Y

7.       S7 27 N N Y Y

8.       S8 24 N N N N

9.       S9 26 N N Y Y

10.     S10 23 N N Y Y

11.     S11 28 N N N N

12.     S12 30 Y Y Y Y

13.     S13 31 N N Y Y

14.     S14 24 N N Y Y

15.     S15 24 Y Y N N

16.     S16 25 N N Y Y

17.     S17 25 N N N N

18.     S18 24 N N Y Y

19.     S19 25 N N Y Y

20.     S20 25 N N Y Y

N: No; Y: Yes; W: Week

 

 

Tape Stripping Method

In Microscopic examination; Site S1 (Blank) slide shows Stratum corneum Cell line as shown in Figure 3. Site S2 (Sample A i.e. Scrub with Polyethylene beads) slide shows very few cells means good exfoliation effect on skin (Figure 4). Site S3 (Sample B i.e. Scrub with Jojoba meal) shows comparable cell lines as Shown in Figure 5; hence it gives mild exfoliation effect.

 

 

Figure 3. Microscopic View of Cell line Separated from Site S1 (Blank)

 

Figure 4. Microscopic View of Cell line Separated from Site S2 (Sample A: Scrub with Polyethylene beads)

 

Figure 5. Microscopic View of Cell line Separated from Site S3 (Sample B: Scrub with Jojoba meal)

 

CONCLUSION:

In conclusion, results of this investigation revealed that Face Scrub with Synthetic exfoliant i.e. Polyethylene beads and Face Scrub with Natural exfoliant i.e. Jojoba meal found stable in stability study.

 

In Subject Study; it found that Face Scrub with Synthetic exfoliant i.e. 2 % Polyethylene beads shows better exfoliation effect compare to Face Scrub with Natural exfoliant i.e. 2% Jojoba meal which give mild exfoliation effect. According to subject response it was found that the Scrub containing polyethylene beads was very well accepted by the people with oily, normal and combination skin as it give good exfoliation which is of prime importance for this product, while the Scrub with jojoba meal also shows mild and gentle exfoliating effects and it could be used for exfoliation of sensitive skin and dry skin which need mild exfoliation.

 

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4.     Government of India. Indian Standard Specification of Toothpaste IS: 6356:2001-Bureau of Indian Standards. Bureau of Indian Standards (Ministry of Consumer Affairs, Food and Public Distribution, Government of India), Manak Bhavan, New Delhi, India. 2001; pp. 1-17.

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Received on 23.03.2016 Accepted on 28.04.2016

A&V Publications all right reserved

Research J. Topical and Cosmetic Sci. 7(1): Jan.-June 2016 page 01-08

DOI: 10.5958/2321-5844.2016.00001.7