Evaluation of Sun Protective Formula (SPF) of Indian Cow Dung as Organic Sunscreen Agent

 

P A Patil1, Monika Kolekar2, Vikranti Koli3, Amruta Kamble4

Dept. of Pharmaceutical Chemistry, Rajarambapu College of Pharmacy, Kasegaon.

*Corresponding Author E-mail: prithvirajpatil87@gmail.com, www.kespharmacy.com

 

ABSTRACT:

The sunlight contains UV electromagnetic light which is very harmful, can cause skin damage, such as sunburn, skin cancer, oxidative damage to skin cells. Various sunscreen formulations available in the market which contains chemicals as active agent and excipients. In this work we evaluated sun protection activity of Indian cow dung as natural and organic sunscreen agent.

 

KEYWORDS: Sunscreen, SPF, Organic, cow dung.

 

 


1. INTRODUCTION:

UV radiation in sunlight is major problem and responsible for various diseases like skin cancer now a days1. In order to block this dangerous and harmful U V radiation an effective sun protective agent is essential2. Now days various sun screen available in the market which contains harmful chemicals which produces skin etching and allergy3. In this study we evaluated cow dung as sun protective organic source. In addition it is abundantly available in village regions of India and very economical4. Further work is needed to make suitable formulation of it.

 

2. MATERIAL AND METHODS:

2.1. Solubility studies:

Cow dung obtained from local places was sun dried and solubility studies were carried out in various solvents like ethanol, distilled water, N-hexane and Acetone. The results of studies are as follow,

 

Sr. No

Distilled water

Ethanol

Acetone

N-hexane

1

Partially soluble

Partially soluble

Freely soluble

Freely soluble

 

2.2 Sample Preparation:

Based on solubility studies samples of concentration 10000 microgram per ml of were prepared in different solvents to determine the absorbance on UV spectrophotometer.

 

2.3 Determination of Absorbance:

The absorbance of different samples was determined on UV spectrophotometer at the various wavelengths 290, 295, 300, 305, 310, 315, 320.

 

2.4 Determination of SPF using Mansur equation:

 

Mansur et al. (1986) developed a very simple mathematical equation which is as follows: In this equation, CF=10 (correction factor), EE (λ)=erythemogenic effect of radiation at wavelength λ,

I (λ)=intensity of solar light at wavelength λ, and abs (λ)=absorbance of wavelength λ by a solution of the preparation. The obtained absorbance values were multiplied by the EE (λ) values; their summation was taken and multiplied by the correction factor 10.

 

 

3. RESULTS AND DISCUSSION:

3.1 Ethanol:

Sr. No

Wavelength

EE (λ). I (λ)

Absorbance

SPF

1

290

0.01

1.676

0.1676

2

295

0.08

1.676

1.3408

3

300

0.28

1.434

4.0152

4

305

0.32

1.154

3.6928

5

310

0.18

0.876

1.5668

6

315

0.08

0.654

0.5232

7

320

0.01

0.567

0.0567

 ∑11.3631

 

3.2 N-hexane

Sr. No

Wavelength

EE (λ). I (λ)

Absorbance

SPF

1

290

0.01

1.705

0.1705

2

295

0.08

1.626

1.3008

3

300

0.28

1.392

3.8976

4

305

032

1.025

3.28

5

310

018

0.695

1.251

6

315

0.08

0.436

0.3488

7

320

0.01

0.271

0.0276

 ∑ 10.2763

 

3.3 Acetone:

Sr.no

Wavelength

EE (λ). I (λ)

Absorbance

SPF

1

290

0.01

1.649

0.1649

2

295

0.08

1.562

1.2496

3

300

0.28

1.329

3.7212

4

305

0.32

0.927

3.1104

5

310

0.18

0.662

1.1916

6

315

0.08

0.418

0.3344

7

320

0.01

0.255

0.0255

 ∑9.7976

 

Fig 1 SPF of cow dung in ethanol

 

Fig.2 SPF of cow dung in acetone

 

Fig.3 SPF of cow dung in N-hexane

 

4. CONCLUSION:

From above results we can conclude that cow dung has sun protection activity which is freely available and organic in nature.

 

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Received on 27.03.2019                    Accepted on 09.05.2019

ŠA&V Publications all right reserved

Research J. Topical and Cosmetic Sci.10(1):07-08.

DOI: 10.5958/2321-5844.2019.00002.5