Inflammation is the self-protection reaction of the body to remove damaging foreign particles and start the healing process. In the body inflammation is act as protective and defense mechanism. Much pathological variations occur inflammatory condition. The production of effective inflammatory mediators is caused by Host proteins or microbial products; such as complement proteins, kinins and coagulation structures, Activated by microorganisms and damaged tissues. Inflammation can classify inflammation as either acute or chronic. Primary aim of the present study was to investigate the possible anti-inflammatory and antibacterial mechanism of Psidium guajava extract using in-vitro model. Ethyl acetate extract of Psidium guajava was evaluated in vitro model by protein denaturation method for anti-inflammatory activity and disk diffusion method for antibacterial activity. The ethyl acetate extract of Psidium guajava significantly inhibited % protein denaturation as compared to standard drug and extract shows promising antibacterial activity against different fungus species. It can be postulated from the observed results the anti-inflammatory and antifungal activity of Psidium guajava could be due to its inhibition of protein denaturation and minimum inhibitory concentration.
Cite this article:
Akshay R. Yadav, Shrinivas K. Mohite. Screening of In-vitro anti-inflammatory and Antifungal assay of Psidium guajava Leaf Extracts. Research J. Topical and Cosmetic Sci. 2020; 11(2):62-64. doi: 10.5958/2321-5844.2020.00011.4
Akshay R. Yadav, Shrinivas K. Mohite. Screening of In-vitro anti-inflammatory and Antifungal assay of Psidium guajava Leaf Extracts. Research J. Topical and Cosmetic Sci. 2020; 11(2):62-64. doi: 10.5958/2321-5844.2020.00011.4 Available on: http://rjtcsonline.com/AbstractView.aspx?PID=2020-11-2-3
1. Ghosh P, Mandal A, Chakraborty P, et al. Triterpenoids from Psidium guajava with biocidal activity. Indian J Pharm Sci. 2010; 72(4):504-507.
2. Qadan F, Thewaini AJ, Ali DA, Afifi R, Elkhawad A, Matalka KZ. The antimicrobial activities of Psidium guajava and Juglans regia leaf extracts to acne-developing organisms. Am J ChinMed. 2005; 33(2):197-204.
3. Chah KF, Eze CA, Emuelosi CE, Esimone CO. Antibacterial and wound healing properties of methanolic extracts of some Nigerian medicinalplants. J Ethnopharmacol. 2006; 104(1-2):164-167.
4. Deo A, Shastri NV. Purification and characterization of polygalacturonase-inhibitory proteins from Psidium guajava Linn. (guava) fruit. Plant Sci. 2003; 164:147-156.
5. Rahim N, Gomes D, Watanabe H. Antibacterial activity of Psidium guajava leaf and bark against multidrug-resistant Vibrio cholerae: implication for cholera control. Jpn J Infect Dis. 2010; 63(4):271-274.
6. Anas K, Jayasree P, Vijayakumar T, Kumar M. In vitro antibacterial activity of Psidium guajava Linn. leaf extract on clinical isolates of multidrug resistant Staphylococcus aureus. Indian J Exp Biol. 2008; 46(1):41-46.
7. Yadav A, Mohite S. Screening of In-vitro anti-inflammatory and Antibacterial assay of Malvastrum Coromandelianum. International Journal of Pharma Sciences and Research. 2020; 11(4): 68-70.
8. Yadav A, Mohite S, Design, Synthesis and Characterization of Some Novel benzamide derivatives and it’s Pharmacological Screening. Int J Sci Res Sci Technol. 2020; 7(2): 68-74.
9. Rajput M. D, Yadav A. R, Mohite S.K. Synthesis, Characterization of Benzimidazole Derivatives as Potent Antimicrobial Agents. Int. J. Pharm., 2020; 17(4): 279-285.
10. Gavarkar PS, Somani RR. Synthesis of novel azole heterocycles with their anti-tubercular and antifungal evaluation. Int J Chem Sci. 2015; 13(1): 432-440.